DYSREGULATION OF Ca2+MOVEMENT IN PLATELETS FROM PATIENTS WITH ACUTE ISCHAEMIC STROKE
| العنوان: | DYSREGULATION OF Ca2+MOVEMENT IN PLATELETS FROM PATIENTS WITH ACUTE ISCHAEMIC STROKE |
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| المؤلفون: | Chi-Ren Huang, Cheng-Hsien Lu, Chung-Ren Jan, Lian-Hui Lee, Yao-Chung Chuang, Chen-Fu Shaw, Wen-Neng Chang, Shang-Der Chen, Nai-Wen Tsai |
| المصدر: | Clinical and Experimental Pharmacology and Physiology. 36:380-385 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Blood Platelets, Male, medicine.medical_specialty, Physiology, chemistry.chemical_compound, Thrombin, Ischemia, Physiology (medical), Internal medicine, Extracellular, Humans, Medicine, Platelet, Calcium Signaling, Stroke, Aged, Pharmacology, Platelet-activating factor, business.industry, Thrombosis, Middle Aged, Platelet Activation, medicine.disease, Surgery, Intracellular signal transduction, Endocrinology, chemistry, Case-Control Studies, Calcium, Female, Arachidonic acid, business, medicine.drug |
| الوصف: | 1. Platelets play a pivotal role during acute ischaemic stroke. An increase in cytosolic Ca(2+) concentrations ([Ca(2+)](i)) triggers intracellular signal transduction, leading to platelet aggregation and thrombosis. In the present study, we examined the differences between platelets from acute ischaemic stroke patients and at-risk controls in terms of the increase in platelet [Ca(2+)](i). 2. Thirty-one patients with acute ischaemic stroke and 27 at-risk controls were enrolled in the present study. Platelet [Ca(2+)](i) was measured using the fluorescent dye fura-2 after stimulation with 100 micromol/L arachidonic acid (AA), 10 micromol/L ADP, 1 micromol/L platelet-activation factor (PAF) and 0.1 U/mL thrombin. 3. Basal [Ca(2+)](i) was higher in the stroke group compared with at-risk controls, irrespective of the presence or absence of extracellular Ca(2+). In Ca(2+)-containing medium, both PAF and ADP, but not AA and thrombin, significantly increased platelet [Ca(2+)](i) in the stroke group compared with the at-risk controls. However, in Ca(2+)-free medium, only PAF significantly increased platelet [Ca(2+)](i) in the stroke group compared with the at-risk controls. Basal [Ca(2+)](i) and PAF-induced platelet [Ca(2+)](i) increases were still higher in the stroke group at the subacute stage than in the at-risk controls. 4. The results of the present study provide direct evidence that Ca(2+) signalling in platelets from acute ischaemic stroke patients was altered in response to particular stimuli. The dysregulation of Ca(2+) movement in platelets may persist up to the subacute stage of ischaemic stroke. |
| تدمد: | 1440-1681 0305-1870 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5a80f45a5f55a0ff685b0873f8d3d05Test https://doi.org/10.1111/j.1440-1681.2008.05068.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f5a80f45a5f55a0ff685b0873f8d3d05 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14401681 03051870 |
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