Functional Outcome after Reconstruction of a Long Nerve Gap in Rabbits Using Optimized Decellularized Nerve Allografts
| العنوان: | Functional Outcome after Reconstruction of a Long Nerve Gap in Rabbits Using Optimized Decellularized Nerve Allografts |
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| المؤلفون: | Steven E.R. Hovius, Caroline A. Hundepool, Liselotte F. Bulstra, Alexander Y. Shin, Allen T. Bishop, Patricia F. Friedrich |
| المساهمون: | Plastic and Reconstructive Surgery and Hand Surgery |
| المصدر: | Plastic and Reconstructive Surgery, 145, 1442-1450 Plastic and Reconstructive Surgery, 145(6), 1442-1450. Lippincott Williams & Wilkins Plastic and Reconstructive Surgery, 145, 6, pp. 1442-1450 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Action Potentials, Motor nerve, Isometric exercise, 030230 surgery, Transplantation, Autologous, 03 medical and health sciences, 0302 clinical medicine, Peripheral Nerve Injuries, medicine, Animals, Humans, Transplantation, Homologous, Autografts, Muscle, Skeletal, Decellularization, Nerve allograft, business.industry, Ultrasound, Peroneal Nerve, Recovery of Function, Allografts, Nerve Regeneration, Surgery, Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10], Cold Temperature, Disease Models, Animal, Electrophysiology, surgical procedures, operative, 030220 oncology & carcinogenesis, Muscle weight, Rabbits, Tissue Preservation, Frozen storage, business |
| الوصف: | Contains fulltext : 220188.pdf (Publisher’s version ) (Closed access) BACKGROUND: Processed nerve allografts are a promising alternative to nerve autografts, providing an unlimited, readily available supply and avoiding donor-site morbidity and the need for immunosuppression. Currently, clinically available nerve allografts do not provide satisfactory results for motor reconstruction. This study evaluated motor recovery after reconstruction of a long nerve gap using a processed nerve allograft and the influence of storage techniques. METHODS: Nerve allografts were decellularized using elastase and detergents and stored at either 4 degrees or -80 degrees C. In 36 New Zealand White rabbits, a 3-cm peroneal nerve gap was repaired with either an autograft (group 1, control) or a cold-stored (group 2) or frozen-stored (group 3) processed nerve allograft. Nerve recovery was evaluated using longitudinal ultrasound measurements, electrophysiology (compound muscle action potentials), isometric tetanic force, wet muscle weight, and histomorphometry after 24 weeks. RESULTS: Longitudinal ultrasound measurements showed that the cold-stored allograft provided earlier regeneration than the frozen-stored allograft. Furthermore, ultrasound showed significantly inferior recovery in group 3 than in both other groups (p < 0.05). Muscle weight and isometric tetanic force showed similar outcomes in the autograft and cold-stored allograft groups [p = 0.096 (muscle weight) and p = 0.286 (isometric tetanic force)], and confirmed the inferiority of the frozen-stored allograft to the autograft [p < 0.01 (muscle weight) and p = 0.02 (isometric tetanic force)]. CONCLUSIONS: Frozen storage of the nerve allograft significantly impairs functional recovery and should be avoided. The cold-stored optimized nerve allograft yields functional recovery similar to the gold standard autograft in the reconstruction of a 3-cm motor nerve defect. Future studies should focus on further improvement of the nerve allograft. |
| وصف الملف: | application/pdf |
| تدمد: | 0032-1052 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3f38a2bf8bab5636fea125851fc9c11Test http://hdl.handle.net/2066/220188Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f3f38a2bf8bab5636fea125851fc9c11 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00321052 |
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