Insulin regulation of gene expression and concentrations of white adipose tissue-derived proteins in vivo in healthy men: relation to adiponutrin
| العنوان: | Insulin regulation of gene expression and concentrations of white adipose tissue-derived proteins in vivo in healthy men: relation to adiponutrin |
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| المؤلفون: | Hubert Vidal, Geneviève Beauregard, May Faraj, Youssef Tahiri, Marthe Moldes, Katherine Cianflone, Karine Clément, Emmanuelle Loizon, Rémi Rabasa-Lhoret |
| المصدر: | Journal of Endocrinology. 191:427-435 |
| بيانات النشر: | Bioscientifica, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Adult, Blood Glucose, Leptin, Male, medicine.medical_specialty, Adipose Tissue, White, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Type 2 diabetes, White adipose tissue, Phosphatidylinositol 3-Kinases, Endocrinology, Hexokinase, Internal medicine, medicine, Hyperinsulinemia, Humans, Insulin, Adiponutrin, education, education.field_of_study, biology, Adiponectin, Membrane Proteins, Complement C3, medicine.disease, Insulin receptor, Glucose, Diabetes Mellitus, Type 2, Gene Expression Regulation, biology.protein, Insulin Resistance |
| الوصف: | Adiponutrin is a newly described white adipose tissue (WAT)-derived protein whose function and regulation remain widely unclear in humans though it is suggested to be related to insulin sensitivity. Recently, we found that adiponutrin expression is reduced in type 2 diabetic subjects in basal and insulin-stimulated states. To examine adiponutrin regulation by the insulin pathway in relation to other WAT-related proteins with well-known relation to insulin signaling and action, we examined in healthy young men (1) the association of adiponutrin with p85α PI3K and HKII, leptin, adiponectin, and acylation-stimulating protein (ASP) and (2) the regulation of adiponutrin and WAT-derived proteins by 3-h hyperinsulinemic euglycemic clamp (HIEG). At baseline (N = 20), adiponutrin expressions were positively correlated with those of p85α PI3K (R = 0.54, P = 0.017), HKII (R = 0.58, P = 0.010), and serum leptin (R = 0.51, P = 0.036), but not with any other parameter measured including insulin sensitivity. Hyperinsulinemia (N = 10, +2365% above baseline) significantly increased the expression of adiponutrin (+770%, P = 0.002), p85α PI3K (+150%, P = 0.033), HKII (+147%, P = 0.007), and serum leptin (+11%, P = 0.031), while it decreased serum adiponectin (−15%, P = 0.001). In the insulin-stimulated state, adiponutrin mRNA expression levels correlated with basal p85α PI3K (R = 0.76, P = 0.018) and HKII (R = 0.86, P = 0.003) expression levels, with percentage increase in insulin (R = 0.73, P = 0.040), and with insulin-stimulated state HKII (R = 0.82, P = 0.007), leptin (R = 0.84, P = 0.005), and adiponectin (R = 0.85, P = 0.004) mRNA levels. In healthy young men, adiponutrin expression is unregulated by hyperinsulinemia and is related to basal and/or insulin-stimulated p85α PI3K, HKII, adiponectin, and leptin expression levels. We hypothesize that insulin-mediated regulation of adiponutrin expression is under the PI3K pathway. The relevance of the present findings to reduced adiponutrin expression in type 2 diabetes is discussed. |
| تدمد: | 1479-6805 0022-0795 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3c9db07b0a1a45091ea13f88283066dTest https://doi.org/10.1677/joe.1.06659Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f3c9db07b0a1a45091ea13f88283066d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14796805 00220795 |
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