Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae amylase-encoding genes
| العنوان: | Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae amylase-encoding genes |
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| المؤلفون: | Toshitaka Minetoki, Chieko Kumagai, Katsuhiko Kitamoto, Kojiro Takahashi, Katsuya Gomi |
| المصدر: | Applied Microbiology and Biotechnology. 50:459-467 |
| بيانات النشر: | Springer Science and Business Media LLC, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Aspergillus oryzae, Genes, Fungal, Molecular Sequence Data, Biology, Applied Microbiology and Biotechnology, Conserved sequence, Fungal Proteins, Gene expression, Promoter Regions, Genetic, Gene, Conserved Sequence, Genetics, Regulation of gene expression, Fungal protein, Base Sequence, Fungal genetics, Gene Expression Regulation, Bacterial, General Medicine, Blotting, Northern, biology.organism_classification, Molecular biology, Glucose, Regulatory sequence, Amylases, Electrophoresis, Polyacrylamide Gel, Transformation, Bacterial, Glucosidases, Biotechnology |
| الوصف: | The role of the conserved sequence region III in the promoter regions of the amylase-encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of multiple copies of the region III fragment into the agdA promoter resulted in a significant increase in promoter activity at the transcriptional level. This result suggests that the fragment comprising region III consists of one or more cis-acting sequence(s). Moreover, expression of the agdA gene under the control of the improved agdA promoter resulted in efficient overproduction of alpha-glucosidase, even in the presence of glucose. Thus, overexpression of genes controlled by the improved promoter incorporating region III is possible. Interestingly, expression of the amyB and glaA genes in the transformant was strongly repressed. This result suggests that the trans-acting regulatory protein(s) that interact with region III are common to these amylase genes and that the titration of regulatory protein(s) reduced the expression of the amyB and glaA genes. |
| تدمد: | 1432-0614 0175-7598 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f22f043969c9dc231bb0718b174021f6Test https://doi.org/10.1007/s002530051321Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....f22f043969c9dc231bb0718b174021f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320614 01757598 |
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