Nine Different Chemical Species and Action Mechanisms of Pancreatic Lipase Ligands Screened Out from Forsythia suspensa Leaves All at One Time
| العنوان: | Nine Different Chemical Species and Action Mechanisms of Pancreatic Lipase Ligands Screened Out from Forsythia suspensa Leaves All at One Time |
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| المؤلفون: | Li Yayun, Liwei Zhang, Tinggui Chen |
| المصدر: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 5; Pages: 795 |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | obesity, Drug Evaluation, Preclinical, Pharmaceutical Science, Ligands, 01 natural sciences, Lignans, Mass Spectrometry, Article, Forsythia suspensa leaves, affinity screening, pancreatic lipase inhibitor, Analytical Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Rutin, Hesperidin, Inhibitory Concentration 50, 0302 clinical medicine, Non-competitive inhibition, Chlorogenic acid, Drug Discovery, Caffeic acid, Humans, Physical and Theoretical Chemistry, Enzyme Inhibitors, Forsythia, Chromatography, High Pressure Liquid, Flavonoids, Forsythia suspensa, biology, Plant Extracts, 010401 analytical chemistry, Organic Chemistry, Polyphenols, Lipase, Phenylethyl Alcohol, biology.organism_classification, 0104 chemical sciences, Plant Leaves, chemistry, Biochemistry, Chemistry (miscellaneous), 030220 oncology & carcinogenesis, Molecular Medicine, Phenethyl alcohol, Uncompetitive inhibitor |
| الوصف: | It is difficult to screen out as many active components as possible from natural plants all at one time. In this study, subfractions of Forsythia suspensa leaves were firstly prepared; then, their inhibitive abilities on pancreatic lipase were tested; finally, the highest inhibiting subfraction was screened by self-made immobilized pancreatic lipase. Results showed that nine ligands, including eight inhibitors and one promotor, were screened out all at one time. They were three flavonoids (rutin, IC50: 149 ± 6.0 μmol/L; hesperidin, 52.4 μmol/L; kaempferol-3-O-rutinoside, isolated from F. suspensa leaves for the first time, IC50 notably reached 2.9 ± 0.5 μmol/L), two polyphenols (chlorogenic acid, 3150 ± 120 μmol/L; caffeic acid, 1394 ± 52 μmol/L), two lignans (phillyrin, promoter; arctigenin, 2129 ± 10.5 μmol/L), and two phenethyl alcohol (forsythiaside A, 2155 ± 8.5 μmol/L; its isomer). Their action mechanisms included competitive inhibition, competitive promotion, noncompetitive inhibition, and uncompetitive inhibition. In sum, using the appropriate methods, more active ingredients can be simply and quickly screened out all at one time from a complex natural product system. In addition, F. suspensa leaves contain numerous inhibitors of pancreatic lipase. |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee9c688b457238be0957fd0ba4b6389eTest https://pubmed.ncbi.nlm.nih.gov/28498356Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ee9c688b457238be0957fd0ba4b6389e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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