Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage
| العنوان: | Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage |
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| المؤلفون: | Masaharu Shin-Ya, S. Tsuchida, S. Shimomura, Tsunao Kishida, Shuji Nakagawa, Hiroaki Inoue, Kuniaki Honjo, Yuji Arai, S. Ichimaru, Osam Mazda, Hiroyoshi Fujiwara, Toshikazu Kubo |
| المصدر: | International Journal of Molecular Sciences; Volume 17; Issue 7; Pages: 1013 International Journal of Molecular Sciences, Vol 17, Iss 7, p 1013 (2016) International Journal of Molecular Sciences |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cartilage, Articular, Male, Anabolism, hypoxia-inducible factor 1 α, Extracellular matrix, lcsh:Chemistry, chemistry.chemical_compound, 0302 clinical medicine, Hyaluronic acid, hyaluronic acid, CD44, Receptor, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, Extracellular Matrix Proteins, General Medicine, Transfection, Cell Hypoxia, Computer Science Applications, Blot, Hyaluronan Receptors, Biochemistry, medicine.symptom, medicine.medical_specialty, extracellular matrix, Type II collagen, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, medicine, Animals, articular cartilage, Physical and Theoretical Chemistry, Rats, Wistar, Molecular Biology, 030203 arthritis & rheumatology, Organic Chemistry, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, Rats, Oxygen, 030104 developmental biology, Endocrinology, chemistry, lcsh:Biology (General), lcsh:QD1-999 |
| الوصف: | Hyaluronic acid (HA) is used clinically to treat osteoarthritis (OA), but its pharmacological effects under hypoxic conditions remain unclear. Articular chondrocytes in patients with OA are exposed to a hypoxic environment. This study investigated whether hypoxia could potentiate the anabolic effects of exogenous HA in rat articular cartilage and whether these mechanisms involved HA receptors. HA under hypoxic conditions significantly enhanced the expression of extracellular matrix genes and proteins in explant culture, as shown by real-time reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and dimethylmethylene blue (DMMB) assays. Staining with Safranin-O and immunohistochemical staining with antibody to type II collagen were also enhanced in pellet culture. The expression of CD44 was increased by hypoxia and significantly suppressed by transfection with siRNAs targeting hypoxia-inducible factor 1 alpha (siHIF-1α). These findings indicate that hypoxia potentiates the anabolic effects of exogenous HA by a mechanism in which HIF-1α positively regulates the expression of CD44, enhancing the binding affinity for exogenous HA. The anabolic effects of exogenous HA may increase as OA progresses. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms17071013 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edb249264e05e3e350745423a2741f09Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....edb249264e05e3e350745423a2741f09 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms17071013 |