Prognostic significance of Capn4 overexpression in intrahepatic cholangiocarcinoma
| العنوان: | Prognostic significance of Capn4 overexpression in intrahepatic cholangiocarcinoma |
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| المؤلفون: | Xin-Rong Yang, Dou-Sheng Bai, Guo-Ming Shi, Jian Zhou, Xiao-Yong Huang, Jia Fan, Ai-Wu Ke, Chi Zhang, Liu-Xiao Yang |
| المصدر: | PLoS ONE, Vol 8, Iss 1, p e54619 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Pathology, lcsh:Medicine, Kaplan-Meier Estimate, Bile Duct Neoplasm, Metastasis, Cholangiocarcinoma, Gastrointestinal Cancers, Basic Cancer Research, lcsh:Science, Intrahepatic Cholangiocarcinoma, Multidisciplinary, biology, Calpain, Cancer Risk Factors, Liver Neoplasms, Cell migration, Middle Aged, Prognosis, Gene Expression Regulation, Neoplastic, Oncology, Lymphatic Metastasis, Hepatocellular carcinoma, Matrix Metalloproteinase 2, Medicine, Female, Research Article, Adult, Lymphatic metastasis, medicine.medical_specialty, Genetic Causes of Cancer, Gastroenterology and Hepatology, Diagnostic Medicine, Cell Line, Tumor, Gastrointestinal Tumors, Calpain small subunit 1, medicine, Humans, Neoplasm Invasiveness, Aged, Cell Proliferation, Neoplasm Staging, Proportional Hazards Models, lcsh:R, Cancers and Neoplasms, Hepatocellular Carcinoma, medicine.disease, digestive system diseases, Bile Ducts, Intrahepatic, Bile Duct Neoplasms, biology.protein, lcsh:Q, Biomarkers, General Pathology |
| الوصف: | BACKGROUND: Calpain small subunit 1 (Capn4) has been shown to correlate with the metastasis/invasion of hepatocellular carcinoma. This study aimed to investigate the role of Capn4 in intrahepatic cholangiocarcinoma (ICC). METHODS: Capn4 expression was measured in 33 ICC tissues by quantitative real-time polymerase chain reaction and western blot. The role of Capn4 in the migration, invasion and proliferation of ICC cells and matrix metalloproteinase 2 (MMP2) expression were assessed after Capn4 depletion by specific small interfering RNA. Capn4 expression was further examined by immunohistochemistry in a tissue microarray consisting of 140 ICC patients and 13 normal liver tissues, and the prognostic role of Capn4 in ICC was evaluated by Kaplan-Meier and Cox regression analyses. RESULTS: Capn4 expression was significantly higher in the ICC tissues compared to the peritumor tissues. Capn4 down-regulation impaired the migration/invasion ability of HCCC-9810 and QBC939 cells in vitro and decreased MMP2 expression. Capn4 overexpression significantly correlated with the presence of lymphatic metastasis of ICC (p = 0.026) and the tumor-node-metastasis (TNM) stage (p = 0.009). The postoperative 2- and 5-year overall survivals in patients with Capn4(low) were higher than those in the Capn4(high) group. The cumulative recurrence rate in patients with Capn4(low) was much lower than in the Capn4(high) group. Multivariate analysis showed that Capn4 overexpression was an independent prognostic marker in ICC. CONCLUSIONS: Capn4 overexpression was implicated in ICC metastasis/invasion, and Capn4 overexpression may be used as a molecular therapeutic target for ICC. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecfc585fa42218264093764453bf1f4dTest http://europepmc.org/articles/PMC3551843?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ecfc585fa42218264093764453bf1f4d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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