Antiplasmodial and Cytotoxic Flavonoids from Pappea capensis (Eckl. & Zeyh.) Leaves
| العنوان: | Antiplasmodial and Cytotoxic Flavonoids from Pappea capensis (Eckl. & Zeyh.) Leaves |
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| المؤلفون: | Nasir Tajuddeen, Heinrich C. Hoppe, Tarryn Swart, Fanie R. van Heerden |
| المصدر: | Molecules, Vol 26, Iss 3875, p 3875 (2021) Molecules |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Plasmodium falciparum, malaria, Pappea capensis, Pharmaceutical Science, Sapindaceae, Pappea, 01 natural sciences, Article, Analytical Chemistry, HeLa, Antimalarials, chemistry.chemical_compound, QD241-441, antiplasmodial, Lactate dehydrogenase, parasitic diseases, Drug Discovery, Humans, HeLa cells, Malaria, Falciparum, Physical and Theoretical Chemistry, Cytotoxicity, biology, Traditional medicine, Cytotoxins, 010405 organic chemistry, Organic Chemistry, Resazurin, biology.organism_classification, 0104 chemical sciences, Plant Leaves, 010404 medicinal & biomolecular chemistry, chemistry, Phytochemical, Chemistry (miscellaneous), flavonoids, Molecular Medicine, Apiaceae |
| الوصف: | Ethnobotanical surveys indicate that the Masai and Kikuyu in Kenya, the Venda in South Africa, and the Gumuz people of Ethiopia use Pappea capensis for the treatment of malaria. The present study aimed to investigate the phytochemical and antiplasmodial properties of the plant leaves. The bioactive compounds were isolated using chromatographic techniques. The structures were established using NMR, HRMS, and UV spectroscopy. Antiplasmodial activity of P. capensis leaf extract and isolated compounds against chloroquine-sensitive 3D7 P. falciparum was evaluated using the parasite lactate dehydrogenase assay. Cytotoxicity against HeLa (human cervix adenocarcinoma) cells was determined using the resazurin assay. The extract inhibited the viability of Plasmodium falciparum by more than 80% at 50 µg/mL, but it was also cytotoxic against HeLa cells at the same concentration. Chromatographic purification of the extract led to the isolation of four flavonoid glycosides and epicatechin. The compounds displayed a similar activity pattern with the extract against P. falciparum and HeLa cells. The results from this study suggest that the widespread use of P. capensis in traditional medicine for the treatment of malaria might have some merits. However, more selectivity studies are needed to determine whether the leaf extract is cytotoxic against noncancerous cells. |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eca3b5bd490b810137f1fb9a241be066Test https://doi.org/10.3390/molecules26133875Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eca3b5bd490b810137f1fb9a241be066 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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