Detection of Kras Gene in Colorectal Cancer Patients through Liquid Biopsy: A Cost-effective Method
| العنوان: | Detection of Kras Gene in Colorectal Cancer Patients through Liquid Biopsy: A Cost-effective Method |
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| المؤلفون: | Saeeda Baig, Hafiz Syed Muhammad Osama Jafri, Shamim Mushtaq |
| المصدر: | Journal of the College of Physicians and Surgeons Pakistan. 31:1174-1178 |
| بيانات النشر: | College of Physicians and Surgeons Pakistan, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Colorectal cancer, Cost-Benefit Analysis, medicine.disease_cause, Gastroenterology, law.invention, Proto-Oncogene Proteins p21(ras), law, Internal medicine, medicine, Humans, Liquid biopsy, Stage (cooking), Gene, Polymerase chain reaction, business.industry, Liquid Biopsy, Cancer, General Medicine, medicine.disease, Cross-Sectional Studies, Cell-free fetal DNA, Mutation, Female, KRAS, Colorectal Neoplasms, business |
| الوصف: | Objective To detect the Kras gene through liquid biopsy, a less invasive technique in diagnosed colorectal cancer patients. Study design Cross-sectional study. Place and duration of study Department of Oncology, Dr. Ziauddin Hospital and Bait-us-Sukoon Cancer Hospital, Karachi, from 2019 to 2020. Methodology Circulating tumor DNA (ctDNA) in colorectal cancer patients was extracted through magnetic bead technique using MagMAX cell free DNA kit (Thermofisher, Uk). The frequency of Kras gene was quantified using a real-time polymerase chain reaction (RT-PCR) assay (qPCR). ANOVA and Chi-square tests were utilised for statistical analysis. Results Mean threshold cycle (CT) of Kras gene showed significantly higher expression 15.6 ± 1.82 (p=0.001) in stage IV CRC cases compared to early stages (19.53 ± 18.223.7 ± 2.9 and 19.8 ± 2.69 of stage 1, 2 and 3, respectively. Similarly, ΔCT mean of Kras gene at stage IV showed significantly higher expression of 2.48 ± 1.40 (0.048), compared to 2.39 ± 0.6, 3.12 ± 0.68 and 3.15 ± 0.41 of stage 1, 2 and 3, respectively. Males (n=40, 55%) showed significant association (p=0.001) with CRC compared to females (n=33, 45%). Categorisation of tumor types within different age groups revealed that colon cancer was more frequent (n=11, 15.1%) in the 41-50 age group, while rectal cancer was more frequent (n= 11, 15.1%) in the 41-50 age group, while rectal cancer was more in the 51-60 age group (n=11, 15.1%). Conclusion Kras gene was detected with significantly increased levels in plasma of CRC patients at advanced stages. This confirms that liquid biopsy can be used to detect Kras gene in ctDNA of CRC patients through a magnetic bead based technique. Key Words: Liquid biopsy, Circulating tumor DNA, KRAS, Colorectal cancer, Real-time polymerase chain reaction. |
| تدمد: | 1681-7168 1022-386X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec25e34af70d88116ca6b89c28e0df75Test https://doi.org/10.29271/jcpsp.2021.10.1174Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ec25e34af70d88116ca6b89c28e0df75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16817168 1022386X |
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