Rapid tumor formation and development of neutrophilia and splenomegaly in nude mice transplanted with human cells expressing human T cell leukemia virus type I or Tax1
| العنوان: | Rapid tumor formation and development of neutrophilia and splenomegaly in nude mice transplanted with human cells expressing human T cell leukemia virus type I or Tax1 |
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| المؤلفون: | Yuetsu Tanaka, Atsushi Jinno, Y Soda, Kunitada Shimotohno, T Akagi, Hiroo Hoshino |
| المصدر: | Leukemia. 14:1467-1476 |
| بيانات النشر: | Springer Science and Business Media LLC, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Cancer Research, Cell Transplantation, Ratón, medicine.medical_treatment, Mice, Nude, Granulocyte, Biology, Virus, Mice, Granulocyte Colony-Stimulating Factor, Tumor Cells, Cultured, medicine, Animals, Humans, Human T-lymphotropic virus 1, Mice, Inbred BALB C, Granulocyte-Macrophage Colony-Stimulating Factor, Gene Products, tax, Hematology, Virology, Molecular biology, Neutrophilia, Hematopoiesis, Granulocyte colony-stimulating factor, medicine.anatomical_structure, Cytokine, Granulocyte macrophage colony-stimulating factor, Neutrophil Infiltration, Oncology, Cell culture, Splenomegaly, Female, medicine.symptom, medicine.drug |
| الوصف: | Human T cell leukemia virus type I (HTLV-I) or its transcriptional trans-activator, Tax1, was introduced into a human osteosarcoma cell line, HOS, and a Moloney murine sarcoma virus-positive HOS cell line, S+L-HOS. These HTLV-I- or Tax1-expressing cells were injected subcutaneously into nude mice to investigate the effects of HTLV-I on their tumorigenicities. HOS cells did not form any tumors even in the presence of HTLV-I or Tax1. S+L-HOS cells did form small tumors in two-thirds of nude mice. Infection of S+L-HOS cells with HTLV-I, or transduction of Tax1 into S+L-HOS cells markedly facilitated the tumor formation, and the tumor-bearing mice showed marked splenomegaly and neutrophilia. Elevated levels of granulocyte colony-stimulating factor (G-CSF) were detected in sera of these mice and also in the culture supernatants of Tax1-expressing human cells, suggesting that G-CSF in the mouse sera was produced by the human cells. In sera of some mice with splenomegaly and neutrophilia, high levels of murine granulocyte-macrophage colony-stimulating factor (mGM-CSF) were observed, suggesting that Tax1 produced by human cells induced mouse cells to produce mGM-CSF. Only S+L-HOS cell lines expressing Tax1 showed high tumorigenicity in nude mice. Thus, this system will be a useful model of tumor formation, splenomegaly and neutrophilia dependent on Tax1. |
| تدمد: | 1476-5551 0887-6924 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebeb2688aaea6bde3d44a2b828fbf45aTest https://doi.org/10.1038/sj.leu.2401844Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebeb2688aaea6bde3d44a2b828fbf45a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765551 08876924 |
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