Autoantibodies against a novel 51 kDa islet antigen and glutamate decarboxylase isoforms in autoimmune polyendocrine syndrome type I
| العنوان: | Autoantibodies against a novel 51 kDa islet antigen and glutamate decarboxylase isoforms in autoimmune polyendocrine syndrome type I |
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| المؤلفون: | Ola Winqvist, Jan Gustafsson, Olle Kämpe, L. A. Velloso, F A Karlsson |
| المصدر: | Diabetologia. 37:61-69 |
| بيانات النشر: | Springer Science and Business Media LLC, 1994. |
| سنة النشر: | 1994 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, Adolescent, Endocrinology, Diabetes and Metabolism, Glutamate decarboxylase, Fluorescent Antibody Technique, Rats, Inbred WF, Biology, medicine.disease_cause, Autoantigens, Autoimmune Diseases, Autoimmunity, Immunoenzyme Techniques, Islets of Langerhans, Antigen, Internal Medicine, medicine, Animals, Humans, Child, Polyendocrinopathies, Autoimmune, Pancreas, B cell, Autoantibodies, Neurons, Autoimmune disease, geography, geography.geographical_feature_category, Glutamate Decarboxylase, Autoantibody, medicine.disease, Islet, Immunohistochemistry, Rats, Isoenzymes, Diabetes Mellitus, Type 1, medicine.anatomical_structure, Autoimmune polyendocrine syndrome, Immunology, Female |
| الوصف: | Beta-cell function and islet cell antibodies were studied in six patients with autoimmune polyendocrine syndrome type I. All suffered from mucocutaneous candidiasis, five had adrenocortical insufficiency and three hypoparathyroidism. All sera contained high titres of antibodies staining islets of Langerhans. Reactivity against glutamate decarboxylase, predominantly the 65 kDa isoform, was detected by immunoprecipitations and Western blots in five of the six sera, and all six sera immunoprecipitated a 51 kDa antigen from [35S]-methionine labelled rat islet cell lysates. No reactivity against this latter antigen was found in sera of patients with Type 1 (insulin-dependent) diabetes mellitus (n = 9), Graves' disease (n = 5), autoimmune gastritis (n = 4), idiopathic Addison's disease (n = 7), or stiff-man syndrome (n = 2). The 51 kDa antigen was also detected by Western blots using homogenates of rat islets and autoimmune polyendocrine syndrome type I patient sera, whereas no such reactivity was found with homogenates of testes, adrenals, small intestine, spleen, exocrine pancreas or brain. Moreover, the 51 kDa antigen was present in the rat insulinoma cell line RINm 5F but not in the SV-40 transformed, monkey kidney cell line COS, when examined by immunoprecipitations of [35S]-methionine labelled cell lysates and by Western blots. None of the patients with autoimmune polyendocrine syndrome type I had symptoms of diabetes and their insulin responses to glucose challenge were normal. The data illustrate that patients with autoimmune polyendocrine syndrome type I present an autoimmune response against islets of Langerhans, which is apparently different from that associated with classic Type 1 diabetes.(ABSTRACT TRUNCATED AT 250 WORDS) |
| تدمد: | 1432-0428 0012-186X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebda37013d775060db42928d375d33dbTest https://doi.org/10.1007/bf00428779Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebda37013d775060db42928d375d33db |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320428 0012186X |
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