In vitro and in silico cytotoxicity of hinokinin-loaded PLGA microparticle systems against tumoral SiHa cells
| العنوان: | In vitro and in silico cytotoxicity of hinokinin-loaded PLGA microparticle systems against tumoral SiHa cells |
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| المؤلفون: | Renato L. T. Parreira, Regiane Godoy de Lima, Renato Pereira Orenha, Flávia Cristina Rodrigues Lisoni, Alexandre Luiz Souto Borges, Rosangela da Silva de Laurentiz, Márcio Luis Andrade e Silva, Thaís B. Picão, FF Santos, Mário Santos, Eduardo F. Molina |
| المساهمون: | Universidade Estadual Paulista (UNESP), Universidade de Franca, Centro Universitário |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| بيانات النشر: | Taylor & Francis, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Natural products, animal structures, Chemistry, Organic Chemistry, technology, industry, and agriculture, inhibition proliferation assay, Biological activity, molecular docking, Plant Science, macromolecular substances, Biochemistry, In vitro, Analytical Chemistry, PLGA, chemistry.chemical_compound, HaCaT, embryonic structures, Biophysics, Cytotoxic T cell, Piper cubeba, Microparticle, Cytotoxicity, IC50 |
| الوصف: | Made available in DSpace on 2022-04-29T08:36:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 This work aimed to synthesize poly (D, L-lactic-co-glycolic acid) (PLGA) microparticles containing hinokinin (HNK) and to evaluate their cytotoxic activity against tumoral SiHa cells and non-tumoral HaCaT cells. Hinokinin was incorporated into PLGA (PLGA-HNK) with an encapsulation efficiency of 84.18 ± 2.32%. PLGA and PLGA-HNK were characterized by SEM microscopy and showed spherical morphology with an average size of ∼3.33. Encapsulation efficiency was determined by a calibration curve using UV-vis spectroscopy. PLGA-HNK more active inhibiting proliferation of SiHa cells (IC50 = 14.68 µM) than free HNK (IC50 = 225.5 µM). In relation to HaCaT cells, PLGA-HNK showed no significant difference compared to the negative control. These results led to an increase in HNK bioavailability and thereby, biological activity. In silico prediction analysis suggests that HNK is cytotoxic against SiHa cells with E6 and MDM2 inhibition as possible main mechanism of action. Departamento de Física e Química Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista Departamento de Biologia e Zootecnia Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista Núcleo de Pesquisa em Ciências Exatas e Tecnológicas Universidade de Franca Centro Universitário Departamento de Física e Química Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista Departamento de Biologia e Zootecnia Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista |
| DOI: | 10.6084/m9.figshare.16937039 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebb2f216b17f2e4d01aea955c39e60c8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebb2f216b17f2e4d01aea955c39e60c8 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.6084/m9.figshare.16937039 |
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