pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting
| العنوان: | pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting |
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| المؤلفون: | Ruben De Coen, Stefaan De Koker, Neil Ayres, Johan Grooten, Vimal Kumar, Serge Van Calenbergh, Lutz Nuhn, Martijn D. P. Risseeuw, Nane Vanparijs, Benoit Louage, Leeanne Taylor, Lien Lybaert, Bruno G. De Geest |
| المصدر: | BIOMACROMOLECULES |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Polymers and Plastics, Polymers, Mannose, Nanogels, 02 engineering and technology, 01 natural sciences, Polyethylene Glycols, Polymerization, chemistry.chemical_compound, Mice, Materials Chemistry, Polyethyleneimine, IN-VIVO, Cells, Cultured, biology, IMMUNE-RESPONSES, RAFT POLYMERIZATION, Raft, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, GLYCOPOLYMERS, DC-SIGN, Chemistry, 0210 nano-technology, Mannose receptor, Mannose Receptor, SYNTHETIC VACCINES, Bioengineering, MANNOSE RECEPTOR, Receptors, Cell Surface, 010402 general chemistry, Endocytosis, Biomaterials, Amphiphile, Polymer chemistry, Animals, Reversible addition−fragmentation chain-transfer polymerization, Lectins, C-Type, CROSS-PRESENTATION, Biology and Life Sciences, Dendritic cell, CATIONIC NANOHYDROGEL PARTICLES, Dendritic Cells, 0104 chemical sciences, Mannose-Binding Lectins, chemistry, biology.protein, Biophysics, POLYMERS |
| الوصف: | We report on the design of glycosylated nanogels via core-cross-linking of amphiphilic non-water-soluble block copolymers composed of an acetylated glycosylated block and a pentafluorophenyl (PFP) activated ester block prepared by reversible addition-fragmentation (RAFT) polymerization. Self-assembly, pH-sensitive core-cross-linking, and removal of remaining PFP esters and protecting groups are achieved in one pot and yield fully hydrated sub-100 nm nanogels. Using cell subsets that exhibit high and low expression of the mannose receptor (MR) under conditions that suppress active endocytosis, we show that mannosylated but not galactosylated nanogels can efficiently target the MR that is expressed on the cell surface of primary dendritic cells (DCs). These nanogels hold promise for immunological applications involving DCs and macrophage subsets. |
| وصف الملف: | application/pdf |
| تدمد: | 1526-4602 1525-7797 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e974e45108717482fd11aadb04bc99eaTest https://pubmed.ncbi.nlm.nih.gov/27231045Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e974e45108717482fd11aadb04bc99ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15264602 15257797 |
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