Evaluation of Caripill™ as a component of platelet storage solution
| العنوان: | Evaluation of Caripill™ as a component of platelet storage solution |
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| المؤلفون: | R. Vani, M. Manasa |
| المصدر: | Hematology, Transfusion and Cell Therapy, Vol 43, Iss 2, Pp 133-140 (2021) Hematology, Transfusion and Cell Therapy Hematology, Transfusion and Cell Therapy, Volume: 43, Issue: 2, Pages: 133-140, Published: 07 JUL 2021 |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Platelets, Antioxidant, medicine.medical_treatment, Storage, 030204 cardiovascular system & hematology, Pharmacology, medicine.disease_cause, Lesion, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lactate dehydrogenase, Immunology and Allergy, Medicine, Platelet, Diseases of the blood and blood-forming organs, biology, business.industry, Carica papaya, Hematology, Platelet transfusion, chemistry, Catalase, Oxidative stress, Caripill™, biology.protein, Original Article, medicine.symptom, RC633-647.5, business, 030215 immunology |
| الوصف: | Background Carica papaya Linn. has high nutraceutical and pharmacological values. The leaves possess antimicrobial, anti-tumor and antioxidant properties. They are used to treat thrombocytopenia during dengue fever and the leaf extract is commercially available as tablets under the name Caripill™ (MicroLabs, Bengaluru). Nevertheless, platelet transfusion is recommended in severe cases of thrombocytopenia, but the platelet storage is limited to 5–7 days at 22−24 °C. Reducing oxidative stress (OS) during platelet storage might help in prolonging the shelf-life, since the OS is known to cause platelet storage lesion. Hence, this study investigated the effects of Caripill™ as an additive in Tyrode’s buffer during extended platelet storage. Methods Platelets isolated from 4 months old male Wistar rats were stored with Caripill™ (50 and 100 μg/ml) at 22 °C for 12 days. Platelet functional and metabolic markers and various OS markers were analyzed on days 0, 4, 8 and 12. Results Caripill™ (50 and 100 μg/ml) maintained platelet functions and lactate dehydrogenase, elevated nitrites, reduced glucose consumption, protected proteins and up-regulated the antioxidant enzymes. However, the CP100 up-regulated catalase from day 4, elevated nitrites from day 8, prevented the formation of secondary products of lipid peroxidation and increased the total antioxidant capacity on day 4. Conclusions Caripill™ reduced platelet storage lesion up to day 8 of storage. Results suggest that a higher concentration of Caripill™ was more effective in combating the oxidative damage during platelet storage. This study throws light on the beneficial effects of Caripill™ in combating oxidative stress during platelet storage. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 2531-1379 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8ae77d824f97733c7af78db783ad65eTest http://www.sciencedirect.com/science/article/pii/S2531137920300237Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e8ae77d824f97733c7af78db783ad65e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25311379 |
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