Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro
| العنوان: | Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro |
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| المؤلفون: | Yao-Lan Li, Xu-Hui Xiao, Man-Mei Li, Wen-Jun Song, Zhong Liu, Wei Tang, Li-Feng Chen, Ling Zhuang, Yu-Bo Zhang, Guo-Cai Wang |
| المصدر: | Journal of Agricultural and Food Chemistry. 65:3481-3489 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Rhodomyrtus tomentosa, Myrtaceae, Inflammation, Respiratory Syncytial Virus Infections, 03 medical and health sciences, medicine, Humans, Interleukin 6, biology, Cyclohexanones, Interleukin-6, Plant Extracts, Tumor Necrosis Factor-alpha, NF-kappa B, General Chemistry, biology.organism_classification, Virology, Molecular biology, In vitro, Respiratory Syncytial Viruses, IκBα, 030104 developmental biology, Real-time polymerase chain reaction, Fruit, biology.protein, Phosphorylation, I-kappa B Proteins, medicine.symptom, Thioredoxin, Reactive Oxygen Species, General Agricultural and Biological Sciences |
| الوصف: | Respiratory syncytial virus (RSV) is one of the most common respiratory pathogens. Immoderate inflammation plays a great role in causing RSV-induced diseases. In the present study, watsonianone A, isolated from the fruit of Rhodomyrtus tomentosa (Ait.) Hassk, was found to show a good inhibitory effect on RSV-induced NO production, with a half-maximal inhibitory concentration of 37.2 ± 1.6 μM. Enzyme-linked immunosorbent assay and fluorescence quantitative polymerase chain reaction analyses indicated that watsonianone A markedly reduced both mRNA and protein levels of tumor necrosis factor α, interleukin 6, and monocyte chemoattractant protein 1 in RSV-infected RAW264.7 cells. Mechanistically, watsonianone A inhibited nuclear factor κB (NF-κB) activation by suppressing IκBα phosphorylation. Further analysis revealed that watsonianone A activated the thioredoxin system and decreased intracellular reactive oxygen species (ROS) levels, which are closely associated with NF-κB activation in RSV-infected cells. These results reveal that watsonianone A can attenuate RSV-induced inflammation via the suppression of ROS-sensitive inflammatory signaling. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e87b5bc6bd5fe859e76b081f2135c7e9Test https://doi.org/10.1021/acs.jafc.7b00537Test |
| رقم الانضمام: | edsair.doi.dedup.....e87b5bc6bd5fe859e76b081f2135c7e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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