Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs
| العنوان: | Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis–infected lungs |
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| المؤلفون: | Angela Rodgers, Maximiliano G. Gutierrez, Antony Fearns, Daniel J. Greenwood, Haibo Jiang |
| المصدر: | PLoS Biology, Vol 18, Iss 12, p e3000879 (2020) PLoS Biology PLoS Biology, 18 (12) |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Fluorescence-lifetime imaging microscopy, Antibiotics, Cell, Antitubercular Agents, Pathology and Laboratory Medicine, Diagnostic Radiology, Mice, White Blood Cells, chemistry.chemical_compound, Animal Cells, Microscopy, Medicine and Health Sciences, Electron Microscopy, Diarylquinolines, Biology (General), Lung, Tomography, Mice, Inbred C3H, biology, Antimicrobials, Radiology and Imaging, General Neuroscience, Methods and Resources, Drugs, Pulmonary Imaging, 3. Good health, Actinobacteria, Intracellular Pathogens, medicine.anatomical_structure, Female, Scanning Electron Microscopy, Cellular Types, Pathogens, General Agricultural and Biological Sciences, Imaging Techniques, QH301-705.5, medicine.drug_class, Immune Cells, Immunology, 030106 microbiology, Neuroimaging, Microbial Sensitivity Tests, Research and Analysis Methods, Microbiology, General Biochemistry, Genetics and Molecular Biology, Mycobacterium tuberculosis, 03 medical and health sciences, Diagnostic Medicine, In vivo, Microbial Control, Fluorescence Imaging, medicine, Animals, Tuberculosis, Pharmacology, Blood Cells, Bacteria, General Immunology and Microbiology, Macrophages, Organisms, Biology and Life Sciences, Cell Biology, biology.organism_classification, Molecular biology, Computed Axial Tomography, Microscopy, Electron, 030104 developmental biology, chemistry, Bedaquiline, Neuroscience |
| الوصف: | Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, we developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type–specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, we found that the anti-tuberculosis (TB) drug bedaquiline (BDQ) is localised not only in foamy macrophages in the lungs during infection but also accumulate in polymorphonuclear (PMN) cells. This study uses correlative light, electron and ion microscopy (CLEIM) in vivo to reveal the intracellular fate of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis, with organelle-level resolution. |
| وصف الملف: | application/application/pdf |
| تدمد: | 1545-7885 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e86f1cbf7c4458b0fe62b3fa72675d57Test https://doi.org/10.1371/journal.pbio.3000879Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e86f1cbf7c4458b0fe62b3fa72675d57 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15457885 |
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