Characterization of astrocytes and microglial cells in the hippocampal CA1 region after transient focal cerebral ischemia in rats treated with Ilexonin A
| العنوان: | Characterization of astrocytes and microglial cells in the hippocampal CA1 region after transient focal cerebral ischemia in rats treated with Ilexonin A |
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| المؤلفون: | Hui-Ying Ye, Guan-Yi Zheng, Ai-Ling Xu, Xiao Dong Chen, Qiong Jiang |
| المصدر: | Neural Regeneration Research, Vol 15, Iss 1, Pp 78-85 (2020) Neural Regeneration Research |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, medicine.medical_specialty, middle cerebral artery occlusion, Ischemia, microglia, hippocampal CA1 region, Hippocampal formation, Neuroprotection, lcsh:RC346-429, astrocytes, ilexonin A, neural stem cell, neuroprotection, transient focal cerebral ischemia, 03 medical and health sciences, 0302 clinical medicine, Developmental Neuroscience, Medicine, lcsh:Neurology. Diseases of the nervous system, Glial fibrillary acidic protein, biology, Microglia, business.industry, Nestin, medicine.disease, Neural stem cell, 030104 developmental biology, medicine.anatomical_structure, nervous system, biology.protein, business, 030217 neurology & neurosurgery, Astrocyte, Research Article |
| الوصف: | Ilexonin A is a compound isolated from the root of Ilex pubescens, a traditional Chinese medicine. Ilexonin A has been shown to play a neuroprotective role by regulating the activation of astrocytes and microglia in the peri-infarct area after ischemia. However, the effects of ilexonin A on astrocytes and microglia in the infarct-free region of the hippocampal CA1 region remain unclear. Focal cerebral ischemia models were established by 2-hour occlusion of the middle cerebral artery in rats. Ilexonin A (20, 40 or 80 mg/kg) was administered immediately after ischemia/reperfusion. The astrocyte marker glial fibrillary acidic protein, microglia marker Iba-1, neural stem cell marker nestin and inflammation markers were detected by immunohistochemistry and western blot assay. Expression levels of tumor necrosis factor-α and interleukin 1β were determined by enzyme linked immunosorbent assay in the hippocampal CA1 tissue. Astrocytes were activated immediately in progressively increasing numbers from 1, 3, to 7 days post-ischemia/reperfusion. The number of activated astrocytes further increased in the hippocampal CA1 region after treatment with ilexonin A. Microglial cells remained quiescent after ischemia/reperfusion, but became activated after treatment with ilexonin A. Ilexonin A enhanced nestin expression and reduced the expression of tumor necrosis factor-α and interleukin 1β in the hippocampus post-ischemia/reperfusion. The results of the present study suggest that ilexonin A has a neuroprotective effect in the hippocampus after ischemia/reperfusion, probably through regulating astrocytes and microglia activation, promoting neuronal stem cell proliferation and reducing the levels of pro-inflammatory factors. This study was approved by the Animal Ethics Committee of the Fujian Medical University Union Hospital, China. |
| اللغة: | English |
| تدمد: | 1673-5374 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e81a6481be907825ed5dff0810997711Test http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=1;spage=78;epage=85;aulast=XuTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e81a6481be907825ed5dff0810997711 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16735374 |
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