Impact of Two Measures of Micrometastatic Disease on Clinical Outcomes in Patients with Newly Diagnosed Ewing Sarcoma: A Report from the Children's Oncology Group
| العنوان: | Impact of Two Measures of Micrometastatic Disease on Clinical Outcomes in Patients with Newly Diagnosed Ewing Sarcoma: A Report from the Children's Oncology Group |
|---|---|
| المؤلفون: | Katherine K. Matthay, Crystal L. Mackall, Phillip Barnette, Richard B. Womer, Steven G. DuBois, Douglas S. Hawkins, Elizabeth Sinclair, Stephen L. Lessnick, Richard Gorlick, Katherine A. Janeway, Jeremy V. Edwards, Donald A. Barkauskas, Kieuhoa T. Vo, Holcombe E. Grier, C. Lorrie Epling, Mark Krailo, Elizabeth McIlvaine |
| المصدر: | Clinical cancer research : an official journal of the American Association for Cancer Research, vol 22, iss 14 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Oncology, Cancer Research, Receptor, IGF Type 1, 0302 clinical medicine, Bone Marrow, Prospective Studies, Child, Cancer, Pediatric, screening and diagnosis, medicine.diagnostic_test, Sarcoma, Detection, Haematopoiesis, medicine.anatomical_structure, Neoplasm Micrometastasis, Child, Preschool, 030220 oncology & carcinogenesis, FLI1, Cohort, Female, Receptor, 4.2 Evaluation of markers and technologies, Adult, medicine.medical_specialty, Adolescent, Pediatric Cancer, Oncology and Carcinogenesis, CD99, Bone Neoplasms, Sarcoma, Ewing, 12E7 Antigen, Article, Flow cytometry, Young Adult, 03 medical and health sciences, Rare Diseases, Clinical Research, Ewing, Internal medicine, medicine, Humans, IGF Type 1, Oncology & Carcinogenesis, Preschool, business.industry, Infant, Newborn, Infant, Newborn, medicine.disease, Occult, 030104 developmental biology, Leukocyte Common Antigens, Bone marrow, business |
| الوصف: | Purpose: Flow cytometry and RT-PCR can detect occult Ewing sarcoma cells in the blood and bone marrow. These techniques were used to evaluate the prognostic significance of micrometastatic disease in Ewing sarcoma. Experimental Design: Newly diagnosed patients with Ewing sarcoma were enrolled on two prospective multicenter studies. In the flow cytometry cohort, patients were defined as “positive” for bone marrow micrometastatic disease if their CD99+/CD45− values were above the upper limit in 22 control patients. In the PCR cohort, RT-PCR on blood or bone marrow samples classified the patients as “positive” or “negative” for EWSR1/FLI1 translocations. The association between micrometastatic disease burden with clinical features and outcome was assessed. Coexpression of insulin-like growth factor-1 receptor (IGF-1R) on detected tumor cells was performed in a subset of flow cytometry samples. Results: The median total bone marrow CD99+CD45− percent was 0.0012% (range 0%–1.10%) in the flow cytometry cohort, with 14 of 109 (12.8%) of Ewing sarcoma patients defined as “positive.” In the PCR cohort, 19.6% (44/225) patients were “positive” for any EWSR1/FLI1 translocation in blood or bone marrow. There were no differences in baseline clinical features or event-free or overall survival between patients classified as “positive” versus “negative” by either method. CD99+CD45− cells had significantly higher IGF-1R expression compared with CD45+ hematopoietic cells (mean geometric mean fluorescence intensity 982.7 vs. 190.9; P < 0.001). Conclusions: The detection of micrometastatic disease at initial diagnosis by flow cytometry or RT-PCR is not associated with outcome in newly diagnosed patients with Ewing sarcoma. Flow cytometry provides a tool to characterize occult micrometastatic tumor cells for proteins of interest. Clin Cancer Res; 22(14); 3643–50. ©2016 AACR. |
| وصف الملف: | application/pdf |
| تدمد: | 1557-3265 1078-0432 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7fc08cb61a8c1bf87bd85c88ca5b61aTest https://doi.org/10.1158/1078-0432.ccr-15-2516Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e7fc08cb61a8c1bf87bd85c88ca5b61a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15573265 10780432 |
|---|