The Roles of Epinephelus coioides miR-122 in SGIV Infection and Replication
| العنوان: | The Roles of Epinephelus coioides miR-122 in SGIV Infection and Replication |
|---|---|
| المؤلفون: | Shaowen Wang, Qiwei Qin, Pin Hong Li, Yu Ling Su, He Jia Chen, Jia Yang He, Hong Yan Sun, Xiaohong Huang, Gang Wang, You Hua Huang |
| المصدر: | Marine Biotechnology (New York, N.y.) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Genes, Viral, medicine.medical_treatment, Epinephelus coioides, Apoptosis, Virus Replication, Applied Microbiology and Biotechnology, Iridovirus, Biological pathway, Fish Diseases, 03 medical and health sciences, 0302 clinical medicine, Transcription (biology), microRNA, medicine, MiR-122, Animals, Grouper, Protease, biology, NF-kappa B, biology.organism_classification, DNA Virus Infections, miR-122, Cell biology, Transcription Factor AP-1, MicroRNAs, SGIV, 030104 developmental biology, Viral replication, 030220 oncology & carcinogenesis, Bass, Original Article |
| الوصف: | In mammals, mature miR-122 is 22 nucleotides long and can be involved in regulating a variety of physiological and biological pathways. In this study, the expression profile and effects of grouper Epinephelus coioides miR-122 response to Singapore grouper iridovirus (SGIV) infection were investigated. The sequences of mature microRNAs (miRNAs) from different organisms are highly conserved, and miR-122 from E. coioides exhibits high similarity to that from mammals and other fish. The expression of miR-122 was up-regulated during SGIV infection. Up-regulation of miR-122 could significantly enhance the cytopathic effects (CPE) induced by SGIV, the transcription levels of viral genes (MCP, VP19, LITAF and ICP18), and viral replication; reduce the expression of inflammatory factors (TNF-a, IL-6, and IL-8), and the activity of AP-1 and NF-κB, and miR-122 can bind the target gene p38α MAPK to regulate the SGIV-induced cell apoptosis and the protease activity of caspase-3. The results indicated that SGIV infection can up-regulate the expression of E. coioides miR-122, and up-regulation of miR-122 can affect the activation of inflammatory factors, the activity of AP-1 and NF-κB, and cell apoptosis to regulate viral replication and proliferation. |
| تدمد: | 1436-2236 1436-2228 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e76c102ba79801ab6196d8a7465f259eTest https://doi.org/10.1007/s10126-021-10023-wTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e76c102ba79801ab6196d8a7465f259e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14362236 14362228 |
|---|