Comprehensive mapping of antigen specific T cell responses in hepatitis C virus infected patients with or without spontaneous viral clearance
| العنوان: | Comprehensive mapping of antigen specific T cell responses in hepatitis C virus infected patients with or without spontaneous viral clearance |
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| المؤلفون: | Zhengkun Tu, Hongqing Yan, Chao Zhang, Xinyue Chen, Junqi Niu, Yonghong Zhang, Jin Zhong, Shasha Wang, Yuanyuan Cui, Rui Hua, Pei Hao, Xia Jin, Dongmei Li |
| المصدر: | PLoS ONE, Vol 12, Iss 2, p e0171217 (2017) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Proteomics, RNA viruses, 0301 basic medicine, Programmed Cell Death 1 Receptor, Epitopes, T-Lymphocyte, lcsh:Medicine, Hepacivirus, Lymphocyte Activation, medicine.disease_cause, Biochemistry, Epitope, White Blood Cells, Database and Informatics Methods, T-Lymphocyte Subsets, Animal Cells, Medicine and Health Sciences, Cytotoxic T cell, Enzyme-Linked Immunoassays, Peptide Libraries, lcsh:Science, Immune Response, Phylogeny, Pathology and laboratory medicine, Multidisciplinary, biology, T Cells, Hepatitis C virus, Antibodies, Monoclonal, virus diseases, Middle Aged, Viral Load, Medical microbiology, Hepatitis C, medicine.anatomical_structure, Viruses, Cytokines, Female, Cellular Types, Pathogens, Antibody, Sequence Analysis, Viral load, Research Article, Adult, Genotype, Bioinformatics, Immune Cells, T cell, Immunology, Sequence Databases, Cytotoxic T cells, Research and Analysis Methods, Antiviral Agents, Peptide Mapping, Microbiology, Young Adult, 03 medical and health sciences, Immune system, medicine, Humans, Immunoassays, Aged, Blood Cells, Flaviviruses, business.industry, lcsh:R, Organisms, Viral pathogens, Biology and Life Sciences, Cell Biology, Virology, Hepatitis viruses, digestive system diseases, Microbial pathogens, Patient Outcome Assessment, Biological Databases, 030104 developmental biology, Epitope mapping, Immunologic Techniques, biology.protein, lcsh:Q, Peptides, business, Epitope Mapping |
| الوصف: | Elucidating protective immunity against HCV is important for the development of a preventative vaccine. We hypothesize that spontaneous resolution of acute HCV infection offers clue to protective immune responses, and that DAA therapy affects the quality and quantity of HCV-specific T cell responses. To test these hypotheses, we performed T cell epitope mapping in 111 HCV-infected individuals including 61 chronically HCV-1b (CHC-1b) infected, 24 chronically HCV-2a (CHC-2a) infected and 26 spontaneously recovered (SPR) patients with 376 overlapping peptides covering the entire HCV polyprotein. Selected T cell epitopes were then used to evaluate T cell responses in another 22 chronically HCV-1b infected patients on DAA therapy. Results showed that SPR had better HCV-specific T cell responses than CHC, as manifested by higher response rate, greater magnitude and broader epitope coverage. In addition, SPR recognized novel epitopes in Core, E1, E2, NS4B, NS5A regions that were not present in the CHC. Furthermore, during the first 24 weeks of DAA therapy, there was no functional immune reconstitution of HCV-specific T cells. These results indicate that T cell responses may be a correlate of protection. Therefore, effective preventative vaccines should elicit a robust T cell response. Although various DAA regimens efficiently cleared viruses from the blood of HCV-infected patients, there was no contemporaneous early T cell immune reconstitution, suggesting that early treatment is needed for preserving the functions of HCV-specific T cells. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6920b57133b679317892f68d4f64220Test http://europepmc.org/articles/PMC5295680?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e6920b57133b679317892f68d4f64220 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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