Treating TB human neuroectodermal cell line with retinoic acid induces the appearance of neuron-like voltage-gated ionic currents
| العنوان: | Treating TB human neuroectodermal cell line with retinoic acid induces the appearance of neuron-like voltage-gated ionic currents |
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| المؤلفون: | Alessandro Martini, Silvia Santillo, Giuseppe Sorrentino, Ezia Guatteo, Nicola Biagio Mercuri, Arianna Polverino |
| المصدر: | Brain research 1711 (2019): 97–105. doi:10.1016/j.brainres.2019.01.019 info:cnr-pdr/source/autori:SilviaSantillo, AlessandroMartini, AriannaPolverino, Nicola B.Mercuri, EziaGuatteo, GiuseppeSorrentino/titolo:Treating TB human neuroectodermal cell line with retinoic acid induces the appearance of neuron-like voltage-gated ionic currents/doi:10.1016%2Fj.brainres.2019.01.019/rivista:Brain research/anno:2019/pagina_da:97/pagina_a:105/intervallo_pagine:97–105/volume:1711 |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Patch-Clamp Techniques, Potassium Channels, Retinoic acid, Tretinoin, Voltage-Gated Sodium Channels, TB cell lineDifferentiationAll-trans retinoic acidVoltage-gated ionic currentsWhole-cell patch clamp, Ion Channels, Sodium Channels, Cell Line, Membrane Potentials, Whole-cell patch clamp, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Meningeal Neoplasms, medicine, Extracellular, Humans, Cytotoxic T cell, Patch clamp, Molecular Biology, Neurons, Voltage-gated ion channel, All-trans retinoic acid, Chemistry, General Neuroscience, Sodium, Cell Differentiation, Depolarization, Molecular biology, 030104 developmental biology, medicine.anatomical_structure, Potassium Channels, Voltage-Gated, Cell culture, Differentiation, Calcium, TB cell line, Voltage-gated ionic currents, Calcium Channels, Neurology (clinical), Neuron, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | TB is a cell line derived from the cerebrospinal fluid sample of a patient with primary leptomeningeal melanomatosis. Our previous immunological and ultrastructural analysis revealed that TB cells differentiate towards a neuronal phenotype when grown in vitro up to 7 days in presence of 10 µM all-trans retinoic acid (RA). Recently, we reported that TB cells are sensitive to the cytotoxic effects of β-amyloid peptides, activating the cytosolic phospholipase A2. To date, it is not known if RA, in addition to inducing morphological changes, also causes functional modification in TB cells, by regulating voltage-gated ionic currents. To this purpose, we performed electrophysiological characterization of undifferentiated (TB) and differentiated (RA-TB) cells by means of whole-cell patch clamp recordings. Upon depolarizing stimuli, both groups displayed voltage-gated K+ outward currents of similar amplitude. By contrast, the low amplitude voltage-gated Na+ currents recorded in undifferentiated TB cells were largely up-regulated by RA exposure. This current was strongly reduced by TTX and lidocaine and completely abolished by removal of extracellular sodium. Furthermore, treatment with RA caused the appearance of a late-onset inward current carried by Ca2+ ions in a subpopulation of TB cells. This current was not affected by removal of extracellular Na+ and was completely blocked by Cd2+, a broad-spectrum blocker of Ca2+ currents. Altogether, our results indicate that RA-differentiation of TB cells induces functional changes by augmenting the amplitude of voltage-gated sodium current and by inducing, in a subpopulation of treated cells, the appearance of a voltage-gated calcium current. |
| تدمد: | 0006-8993 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e506a551060729c923627054ab2a651bTest https://doi.org/10.1016/j.brainres.2019.01.019Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....e506a551060729c923627054ab2a651b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00068993 |
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