Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney
| العنوان: | Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney |
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| المؤلفون: | Anne C. Van Erp, Bente Jespersen, Haiyun Qi, Nichlas Riise Jespersen, Marie Vognstoft Hjortbak, Henri G. D. Leuvenink, Janneke Wiersema-Buist, Petra J. Ottens, Christoffer Laustsen, Rikke Nørregaard, Michael Pedersen |
| المساهمون: | Groningen Institute for Organ Transplantation (GIOT) |
| المصدر: | American Journal of Transplantation, 20(9), 2425-2436. Wiley van Erp, A C, Qi, H, Jespersen, N R, Hjortbak, M V, Ottens, P J, Wiersema-Buist, J, Nørregaard, R, Pedersen, M, Laustsen, C, Leuvenink, H G D & Jespersen, B 2020, ' Organ-specific metabolic profiles of the liver and kidney during brain death and afterwards during normothermic machine perfusion of the kidney ', American Journal of Transplantation, vol. 20, no. 9, pp. 2425-2436 . https://doi.org/10.1111/ajt.15885Test American Journal of Transplantation |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Nephrology, In vivo magnetic resonance spectroscopy, basic (laboratory) research/science, translational research/science, donors and donation, graft survival, basic (laboratory) research, kidney transplantation/nephrology, 030230 surgery, Kidney, GLUCOSE, 0302 clinical medicine, Basic Science, MAGNETIC-RESONANCE, Immunology and Allergy, Pharmacology (medical), donation after brain death (DBD) [donors and donation], donors and donation: donation after brain death (DBD), science, dysfunction, liver transplantation, Organ Preservation, animal models, Perfusion, medicine.anatomical_structure, Liver, Metabolome, Original Article, Brain Death, medicine.medical_specialty, liver allograft function/dysfunction, liver allograft function, kidney (allograft) function, nephrology, kidney transplantation, organ procurement and allocation, kidney (allograft) function/dysfunction, DONOR, 03 medical and health sciences, Internal medicine, medicine, Animals, RENAL VIABILITY, donation after brain death (DBD), Machine perfusion, business.industry, TRANSPLANTATION, REDOX STATE, GRAFT, HYPERPOLARIZED C-13, GLUCONEOGENESIS, Rats, Transplantation, Endocrinology, Gluconeogenesis, translational research, hepatology, ORIGINAL ARTICLES, business, liver transplantation/hepatology, Ex vivo, KETONE-BODY RATIO |
| الوصف: | We investigated metabolic changes during brain death (BD) using hyperpolarized magnetic resonance (MR) spectroscopy and ex vivo graft glucose metabolism during normothermic isolated perfused kidney (IPK) machine perfusion. BD was induced in mechanically ventilated rats by inflation of an epidurally placed catheter; sham‐operated rats served as controls. Hyperpolarized [1‐13C]pyruvate MR spectroscopy was performed to quantify pyruvate metabolism in the liver and kidneys at 3 time points during BD, preceded by injecting hyperpolarized[1‐13C]pyruvate. Following BD, glucose oxidation was measured using tritium‐labeled glucose (d‐6‐3H‐glucose) during IPK reperfusion. Quantitative polymerase chain reaction and biochemistry were performed on tissue/plasma. Immediately following BD induction, lactate increased in both organs (liver: eµd0.21, 95% confidence interval [CI] [−0.27, −0.15]; kidney: eµd0.26, 95% CI [−0.40, −0.12]. After 4 hours of BD, alanine production decreased in the kidney (eµd0.14, 95% CI [0.03, 0.25], P Differences in glucose metabolism in the liver and kidney can be visualized in vivo during brain death using hyperpolarized magnetic resonance spectroscopy and ex vivo following brain death during normothermic kidney machine perfusion using glucose oxidation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1600-6135 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4abebbb1c8300c4b0e4a5afa8b47010Test https://research.rug.nl/en/publications/0ce1a4b1-920f-4e2d-b103-eff80909407aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e4abebbb1c8300c4b0e4a5afa8b47010 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16006135 |
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