Distribution of total DNA and cccDNA in serum and PBMCs may reflect the HBV immune status in HBsAg+ and HBsAg- patients coinfected or not with HIV or HCV
| العنوان: | Distribution of total DNA and cccDNA in serum and PBMCs may reflect the HBV immune status in HBsAg+ and HBsAg- patients coinfected or not with HIV or HCV |
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| المؤلفون: | Paul Carrier, Isabelle Fouchard-Hubert, Françoise Lunel, Véronique Loustaud-Ratti, Annie Lefebvre, François Denis, V. Marczuk, Armand Abergel, A. Wagner, Si Nafa Si Ahmed, M. Debette-Gratien, Sophie Alain, Annick Rousseau, Isabelle Chemin |
| المساهمون: | Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hémodynamique, Interaction Fibrose et Invasivité tumorales Hépatiques (HIFIH), Université d'Angers (UA), Pharmacologie des Immunosuppresseurs et de la Transplantation (PIST), Université de Limoges (UNILIM)-CHU Limoges-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Clinics and Research in Hepatology and Gastroenterology Clinics and Research in Hepatology and Gastroenterology, Elsevier, 2013, 37 (4), pp.373-383. ⟨10.1016/j.clinre.2012.11.002⟩ |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | HBsAg, Hepatitis B virus, [SDV]Life Sciences [q-bio], Population, HIV Infections, Peripheral blood mononuclear cell, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Humans, education, 030304 developmental biology, Retrospective Studies, 0303 health sciences, education.field_of_study, Hepatitis B Surface Antigens, Hepatology, biology, business.industry, Coinfection, Gastroenterology, virus diseases, Retrospective cohort study, cccDNA, medicine.disease, Hepatitis B, Virology, digestive system diseases, 3. Good health, Immunology, DNA, Viral, biology.protein, Leukocytes, Mononuclear, 030211 gastroenterology & hepatology, Antibody, DNA, Circular, business |
| الوصف: | International audience; BackgroundThe potential reservoir role of serum and peripheral blood mononuclear cells (PBMCs) for total HBV DNA (tDNA) and cccDNA still remains unknown.Material and methodsWe analyzed tDNA and cccDNA with a single sensitive and validated standardized real-time PCR method in serum and PBMCs in two populations of chronic HBV infection coinfected or not with HCV and/or HIV viruses: a retrospective cohort of 130 HBsAg-negative (HBsAg−) patients with “anti-HBc alone” or anti-HBc and anti-HBs antibodies (Ab) and a cohort of 70 HBsAg-positive patients, 16 of them being prospectively followed under treatment.ResultsAmong HBsAg− patients, HBV DNA was detected in serum or PBMCs in about half of the cases with various distributions of tDNA and cccDNA: in HIV-negative patients with an “antiHBc alone” profile, tDNA was mostly detected in PBMCs suggesting a possible active role of PBMCs; although cccDNA was not detected in PBMCs in HIV-positive patients, tDNA and cccDNA were mostly observed in serum, suggesting a specific pattern of more “persistent” than “occult” infection in this population. Patients with anti-HBc and anti-HBs Ab harbored tDNA in serum or in PBMCs, regardless of their HIV or HCV status, raising the question of a viral reactivation risk during immunosupression in these patients. Among HBsAg+ patients, tDNA was detected in serum and PBMCs of 88.5% of the cases and cccDNA in 22%. Levels of tDNA in both compartments were highly correlated during treatment, suggesting a passive reservoir role for PBMCs.ConclusionThe respective distribution of tDNA and cccDNA in serum and PBMCs may reflect the different immune statuses of the host in HBsAg+ and HBsAg− patients. The frequency of HBV DNA in PBMCs from AgHBs− patients suggests a viral reactivation risk during immunodepression in those patients. |
| تدمد: | 2210-741X 2210-7401 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e41a1f12fb25bea75823a05b3ce47d43Test https://pubmed.ncbi.nlm.nih.gov/23477988Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....e41a1f12fb25bea75823a05b3ce47d43 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2210741X 22107401 |
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