MSI1 Promotes the Expression of the GBM Stem Cell Marker CD44 by Impairing miRNA-Dependent Degradation
| العنوان: | MSI1 Promotes the Expression of the GBM Stem Cell Marker CD44 by Impairing miRNA-Dependent Degradation |
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| المؤلفون: | Jacob Haase, Rebecca Pötschke, Luiz O. F. Penalva, Sebastian Simmermacher, Claudia Misiak, Markus Glaß, Stefan Hüttelmaier, Caspar D. Kühnöl |
| المصدر: | Cancers Volume 12 Issue 12 Cancers, Vol 12, Iss 3654, p 3654 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, MSI1, cancer stem cell, recurrence, Cell, Stem cell marker, urologic and male genital diseases, lcsh:RC254-282, GBM, Article, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Cancer stem cell, microRNA, medicine, Progenitor cell, CD44, luteolin, miRNA, biology, urogenital system, Neurogenesis, glioblastoma, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, nervous system diseases, 030104 developmental biology, medicine.anatomical_structure, Musashi1, Oncology, 030220 oncology & carcinogenesis, Cancer research, biology.protein |
| الوصف: | The stem cell marker Musashi1 (MSI1) is highly expressed during neurogenesis and in glioblastoma (GBM). MSI1 promotes self-renewal and impairs differentiation in cancer and non-malignant progenitor cells. However, a comprehensive understanding of its role in promoting GBM-driving networks remains to be deciphered. We demonstrate that MSI1 is highly expressed in GBM recurrences, an oncologist&rsquo s major defiance. For the first time, we provide evidence that MSI1 promotes the expression of stem cell markers like CD44, co-expressed with MSI1 within recurrence-promoting cells at the migrating front of primary GBM samples. With GBM cell models of pediatric and adult origin, including isolated primary tumorspheres, we show that MSI1 promotes stem cell-like characteristics. Importantly, it impairs CD44 downregulation in a 3&prime UTR- and miRNA-dependent manner by controlling mRNA turnover. This regulation is disturbed by the previously reported MSI1 inhibitor luteolin, providing further evidence for a therapeutic target potential of MSI1 in GBM treatment. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6694 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3c7e571a2ca1edde1451616dec3767bTest http://europepmc.org/articles/PMC7762192Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e3c7e571a2ca1edde1451616dec3767b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726694 |
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