Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome
| العنوان: | Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome |
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| المؤلفون: | Luis Quintales, Rebeca Serrano, Alicia García, Mar Sánchez, Enrique Vázquez, Francisco Antequera, Sara Serrate González |
| المساهمون: | Ministerio de Economía y Competitividad (España) |
| المصدر: | Nucleus Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Cold Spring Harbor Laboratory Press, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Genómica, hibridación genómica comparativa, ADN, nucleosomas, Computational biology, Saccharomyces cerevisiae, Regulatory Sequences, Nucleic Acid, Genome, DNA sequencing, 03 medical and health sciences, chemistry.chemical_compound, Open Reading Frames, Instituto de Biología Funcional y Genómica, Schizosaccharomyces, evolution, Genetics, Nucleosome, genome organization, Nucleosomal organization, Gene, Genetics (clinical), Comparative Genomic Hybridization, biology, Extra View, nucleosome, DNA, biology.organism_classification, Noncoding DNA, Chromatin, Nucleosomes, 030104 developmental biology, chemistry, base composition, chromatin, Schizosaccharomyces and in Saccharomyces cerevisiae, Genome, Fungal |
| الوصف: | In the yeast genome, a large proportion of nucleosomes occupy well-defined and stable positions. While the contribution of chromatin remodelers and DNA binding proteins to maintain this organization is well established, the relevance of the DNA sequence to nucleosome positioning in the genome remains controversial. Through quantitative analysis of nucleosome positioning, we show that sequence changes distort the nucleosomal pattern at the level of individual nucleosomes in three species of Schizosaccharomyces and in Saccharomyces cerevisiae. This effect is equally detected in transcribed and nontranscribed regions, suggesting the existence of sequence elements that contribute to positioning. To identify such elements, we incorporated information from nucleosomal signatures into artificial synthetic DNA molecules and found that they generated regular nucleosomal arrays indistinguishable from those of endogenous sequences. Strikingly, this information is speciesspecific and can be combined with coding information through the use of synonymous codons such that genes from one species can be engineered to adopt the nucleosomal organization of another. These findings open the possibility of designing coding and noncoding DNA molecules capable of directing their own nucleosomal organization. This work was funded by grant BFU2014-52143-P from the Spanish Ministerio de Economía y Competitividad (MINECO). |
| تدمد: | 1549-5469 1088-9051 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e32d67ef98a71d7e7833eea2794fb792Test http://hdl.handle.net/10261/157058Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e32d67ef98a71d7e7833eea2794fb792 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15495469 10889051 |
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