MK615 Suppresses Hypoxia Tolerance by Up-regulation of E-cadherin in Colorectal Cancer Cells With Mutant KRAS
| العنوان: | MK615 Suppresses Hypoxia Tolerance by Up-regulation of E-cadherin in Colorectal Cancer Cells With Mutant KRAS |
|---|---|
| المؤلفون: | Senji Shirasawa, Yasuko Hashiguchi, Motohiro Sawatsubashi, Kensuke Nishi, Yoshinori Uchida, Takayuki Sueta, Toshifumi Sakata, Anthony Swain, Takafumi Yamano, Toshiyuki Tsunoda |
| المصدر: | Anticancer Research. 40:4687-4694 |
| بيانات النشر: | Anticancer Research USA Inc., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Transcriptional Activation, Cancer Research, Colorectal cancer, Mutant, Biology, medicine.disease_cause, Proto-Oncogene Proteins p21(ras), 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Antigens, CD, Cell Line, Tumor, medicine, Humans, Cell Proliferation, Plant Extracts, Cadherin, General Medicine, Hypoxia (medical), Cadherins, HCT116 Cells, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, biology.organism_classification, Cell Hypoxia, Up-Regulation, Blot, Oncology, 030220 oncology & carcinogenesis, Colonic Neoplasms, Cancer research, Japanese Apricot, Prunus, KRAS, medicine.symptom, Colorectal Neoplasms |
| الوصف: | Background/aim The Japanese apricot "Prunus mume" is a traditional Japanese medicine. MK615, a compound extract from Prunus mume has been reported to have anti-tumor effects. Herein, we used 3D floating (3DF) culture to evaluate the anticancer effects of MK615 against human colorectal cancer (CRC) cells that contain mutant (mt) KRAS. Materials and methods HKe3 cells exogenously expressing mtKRAS (HKe3-mtKRAS) were treated with MK615 in 3DF cultures. The protein levels of hypoxia-inducible factor 1 (HIF-1) and E-cadherin were quantified by western blotting. Results MtKRAS enhanced hypoxia tolerance via up-regulation of HIF-1. The expression of HIF-1 protein was suppressed by constitutive overexpression of E-cadherin in CRC HCT116 spheroids. MK615 increased the expression of E-cadherin and decreased the expression of HIF-1 in HKe3-mtKRAS. These results suggest that MK615 suppresses hypoxia tolerance by up-regulation of E-cadherin in CRC cells with mtKRAS. Conclusion MK615 exhibits properties useful for the potential treatment of CRC patients with mtKRAS. |
| تدمد: | 1791-7530 0250-7005 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1b2b8ec3efd075c57048d149f23f6c3Test https://doi.org/10.21873/anticanres.14468Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e1b2b8ec3efd075c57048d149f23f6c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
|---|