Molecular Cloning and Characterization of a Transcriptional Activator Gene,amyR, Involved in the Amylolytic Gene Expression inAspergillus oryzae
| العنوان: | Molecular Cloning and Characterization of a Transcriptional Activator Gene,amyR, Involved in the Amylolytic Gene Expression inAspergillus oryzae |
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| المؤلفون: | Kenji Ozeki, Toshitaka Minetoki, Chieko Kumagai, Terumi Akeno, Yuzuru Iimura, Naoto Okazaki, Katsuya Gomi |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 64:816-827 |
| بيانات النشر: | Oxford University Press (OUP), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Glycoside Hydrolases, Transcription, Genetic, Aspergillus oryzae, Genes, Fungal, Molecular Sequence Data, Gene Expression, Molecular cloning, Applied Microbiology and Biotechnology, Biochemistry, Gene Expression Regulation, Enzymologic, Analytical Chemistry, Fungal Proteins, chemistry.chemical_compound, Gene Expression Regulation, Fungal, Gene expression, Amino Acid Sequence, Cloning, Molecular, DNA, Fungal, Molecular Biology, Gene, Transcription factor, chemistry.chemical_classification, Genetics, Base Sequence, biology, Organic Chemistry, Titrimetry, General Medicine, Maltose, biology.organism_classification, Artificial Gene Fusion, Enzyme, Lac Operon, chemistry, Trans-Activators, DNA, Transcription Factors, Biotechnology |
| الوصف: | A gene, designated amyR, coding for a transcriptional activator involved in amylolytic gene expression has been cloned from Aspergillus oryzae by screening for a clone that enabled to reverse the reduced expression of the alpha-amylase gene (amyB) promoter. amyR encodes 604 amino acid residues of a putative DNA-binding protein carrying a zinc binuclear cluster motif (Zn(II)2Cys6) belonging to the GAL4 family of transcription factors. The amyR gene disruptants showed a significant restricted growth on starch medium and produced little of the amylolytic enzymes including alpha-amylase and glucoamylase compared with a non-disruptant, indicating that amyR is a transcriptional activator gene involved in starch/maltose-induced efficient expression of the amylolytic genes in A. oryzae. In addition, sequencing analysis found that amyR, agdA (encoding alpha-glucosidase), and amyA (encoding alpha-amylase), are clustered on a 12-kb DNA fragment of the largest chromosome in A. oryzae, and that amyR is about 1.5 kb upstream of agdA and transcribed in the opposite direction. Furthermore, transcriptional analysis revealed that the amyR gene was expressed in the presence of glucose comparable to the level in the presence of maltose, while the amylolytic genes were transcribed at high levels only in the presence of maltose. |
| تدمد: | 1347-6947 0916-8451 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e02d2847343a72cfcfc7721ec5b65abdTest https://doi.org/10.1271/bbb.64.816Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e02d2847343a72cfcfc7721ec5b65abd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13476947 09168451 |
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