The effect of hemolysis on plasma oxidation and nitration in patients with sickle cell disease
| العنوان: | The effect of hemolysis on plasma oxidation and nitration in patients with sickle cell disease |
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| المؤلفون: | Gamze Çelmeli, Mutay Aslan, Alphan Kupesiz, Bülent Antmen, Serdar Dogan |
| المساهمون: | Çukurova Üniversitesi |
| المصدر: | Free Radical Research. 46:883-890 |
| بيانات النشر: | Informa UK Limited, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Adult, Erythrocyte Indices, Male, medicine.medical_specialty, Reticulocytes, Adolescent, Hemoglobin, Sickle, Anemia, Sickle Cell, Dinoprost, Hemolysis, Biochemistry, Plasma, chemistry.chemical_compound, Reticulocyte Count, Lactate dehydrogenase, Internal medicine, Oxidation, medicine, Humans, Child, Mean corpuscular volume, Haptoglobins, L-Lactate Dehydrogenase, medicine.diagnostic_test, biology, Sickle cell disease, Nitrotyrosine, Haptoglobin, Haemolysis, Bilirubin, Hemoglobin A, Nitric oxide, General Medicine, medicine.disease, Blood proteins, Isoenzymes, Endocrinology, chemistry, Child, Preschool, biology.protein, Tyrosine, Female, Hemoglobin, Oxidation-Reduction, Biomarkers |
| الوصف: | PubMedID: 22509726 This study aimed to determine the effect of haemolysis on plasma oxidation and nitration in sickle cell disease (SCD) patients. Blood was collected from haemoglobin (Hb)A volunteers and homozygous HbSS patients who had not received blood transfusions in the last 3 months. Haemolysis was characterised by low levels of haemoglobin and haptoglobin and high levels of reticulocyte, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), plasma cell-free haemoglobin, bilirubin, total lactate dehydrogenase (LDH) and dominance of LDH-1 isoenzyme. Plasma 8-isoprostane, protein carbonyl and nitrotyrosine levels were measured to evaluate oxidised lipids, oxidised and nitrated proteins, respectively. Plasma nitritenitrate levels were also determined to assess nitric oxide (NO) production in both SCD patients and controls. Markers of haemolysis were significantly evident in SCD patients compared to controls. Plasma 8-isoprostane, protein carbonyl and nitrotyrosine levels were markedly elevated in SCD patients compared to controls. Linear regression analysis revealed a significant inverse correlation between haemoglobin and reticulocyte counts and a significant positive correlation of plasma cell-free haemoglobin with protein carbonyl and nitrotyrosine levels. The obtained data shows that increased haemolysis in SCD increases plasma protein oxidation and nitration. © 2012 Informa UK, Ltd. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. This work was supported by a grant (No: 2010.01.0103.007) from Akdeniz University Research Foundation. |
| تدمد: | 1029-2470 1071-5762 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df82e6fbf4f6973a223308d0a3caee6aTest https://doi.org/10.3109/10715762.2012.686037Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....df82e6fbf4f6973a223308d0a3caee6a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10292470 10715762 |
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