Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans
| العنوان: | Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans |
|---|---|
| المؤلفون: | Thierry Azoulay, Zoltán Nagy, Federico Esposti, Hendrik P. N. Scholl, Arnold Szabo, Cameron S. Cowan, Pascal W. Hasler, Santiago B. Rompani, Kun-Chao Wu, Xiao-Long Fang, Ákos Lukáts, Tamas Szikra, Péter Hantz, Lue Xiang, Zi-Bing Jin, Rozina I. Hajdú, Josephine Juettner, Arjun Bharioke, Claudia P Patino-Alvarez, Jacek Krol, Dirk Schübeler, János Németh, Brigitte Gross-Scherf, Akos Kusnyerik, Rei K. Morikawa, Rong-Han Wu, Botond Roska, Arnaud R Krebs, Oezkan Keles, Dominik Hartl, David Goldblum |
| المساهمون: | Jüttner, J., Szabo, A., Gross-Scherf, B., Morikawa, R. K., Rompani, S. B., Hantz, P., Szikra, T., Esposti, F., Cowan, C. S., Bharioke, A., Patino-Alvarez, C. P., Keles, Ö., Kusnyerik, A., Azoulay, T., Hartl, D., Krebs, A. R., Schübeler, D., Hajdu, R. I., Lukats, A., Nemeth, J., Nagy, Z. Z., Wu, K., -C., R., -H., Xiang, L., Fang, X., -L., Jin, Z., -B., Goldblum, D., Hasler, P. W., Scholl, H. P. N., Krol, J., Roska, B. |
| المصدر: | Nature Neuroscience |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cell type, Genetic enhancement, Stimulation, Biology, Gene delivery, Retina, Viral vector, Mice, 03 medical and health sciences, 0302 clinical medicine, In vivo, medicine, Animals, Humans, Promoter Regions, Genetic, Gene, Neurons, General Neuroscience, Gene Transfer Techniques, Dependovirus, In vitro, Cell biology, Mice, Inbred C57BL, Macaca fascicularis, 030104 developmental biology, medicine.anatomical_structure, Gene Targeting, Neuroglia, Neuroscience, 030217 neurology & neurosurgery |
| الوصف: | SummaryTargeting genes to specific neuronal or glial cell types is valuable both for understanding and for repairing brain circuits. Adeno-associated viral vectors (AAVs) are frequently used for gene delivery, but targeting expression to specific cell types is a challenge. We created a library of 230 AAVs, each with a different synthetic promoter designed using four independent strategies. We show that ~11% of these AAVs specifically target expression to neuronal and glial cell types in the mouse retina, mouse brain, non-human primate retinain vivo, and in the human retinain vitro. We demonstrate applications for recording, stimulation, and molecular characterization, as well as the intersectional and combinatorial labeling of cell types. These resources and approaches allow economic, fast, and efficient cell-type targeting in a variety of species, both for fundamental science and for gene therapy. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dece259d455bb946c5b268e7ec2f331dTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dece259d455bb946c5b268e7ec2f331d |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |