Systemic tryptophan and kynurenine catabolite levels relate to severity of rhinovirus-induced asthma exacerbation: a prospective study with a parallel-group design
| العنوان: | Systemic tryptophan and kynurenine catabolite levels relate to severity of rhinovirus-induced asthma exacerbation: a prospective study with a parallel-group design |
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| المؤلفون: | van der Sluijs, K. F., van de Pol, M. A., Kulik, W., Dijkhuis, A., Smids, B. S., van Eijk, H. W., Karlas, J. A., Molenkamp, R., Wolthers, K. C., Johnston, S. L., van der Zee, J. S., Sterk, P. J., Lutter, R., Berger, M., Fens, N., Yick, D., Wagener, A. H., Amelink, M., Reesink, H. J., Bresser, P., Kunst, P. W. A., Venekamp, L. N., Majoor, C. J., van Steenwijk, R. P., Jonkers, R. E. |
| المساهمون: | AII - Amsterdam institute for Infection and Immunity, Intensive Care Medicine, Pulmonology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Laboratory Genetic Metabolic Diseases, Experimental Immunology, Other departments, Medical Microbiology and Infection Prevention, Graduate School |
| المصدر: | Thorax Thorax, 68(12), 1122-1130. BMJ Publishing Group |
| بيانات النشر: | BMJ, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Rhinovirus, medicine.disease_cause, chemistry.chemical_compound, 0302 clinical medicine, ortho-Aminobenzoates, Exhaled breath condensate, Prospective Studies, Kynurenine, 0303 health sciences, Eosinophil cationic protein, medicine.diagnostic_test, Tryptophan, Interleukin, respiratory system, 3. Good health, Breath Tests, Disease Progression, Cytokines, Female, Bronchoalveolar Lavage Fluid, Adult, Pulmonary and Respiratory Medicine, Nitric Oxide, Young Adult, 03 medical and health sciences, medicine, Humans, Indoleamine-Pyrrole 2,3,-Dioxygenase, Peroxidase, 030304 developmental biology, Asthma, Picornaviridae Infections, business.industry, Eosinophil Cationic Protein, Quinolinic Acid, medicine.disease, respiratory tract diseases, Eosinophils, Bronchoalveolar lavage, chemistry, Case-Control Studies, Immunology, business, Biomarkers, 030215 immunology, Quinolinic acid |
| الوصف: | Background Patients with allergic asthma have exacerbations which are frequently caused by rhinovirus infection. The antiviral tryptophan-catabolising enzyme indoleamine 2,3-dioxygenase (IDO) is induced by interferon-γ and suppressed by Th2 mediators interleukin (IL)-4 and IL-13. We hypothesised that local IDO activity after viral airway infection is lower in patients with allergic asthma than in healthy controls. Objective To determine whether IDO activity differs between patients with allergic asthma and healthy individuals before and after rhinovirus infection. Methods Healthy individuals and patients with allergic asthma were experimentally infected with low-dose (10 TCID50) rhinovirus 16. Blood, bronchoalveolar lavage fluid and exhaled breath condensate (for mass spectrometry by UPLC-MS/MS) were obtained before and after rhinovirus challenge. Results IDO activity was not induced by rhinovirus infection in either group, despite increases in cold scores. However, baseline pulmonary IDO activity was lower in patients with allergic asthma than in healthy individuals. In contrast, systemic tryptophan and its catabolites were markedly higher in patients with allergic asthma. Moreover, systemic quinolinic acid and tryptophan were associated with eosinophil cationic protein (r=0.43 and r=0.78, respectively) and eosinophils (r=0.38 and r=0.58, respectively) in bronchoalveolar lavage fluid and peak asthma symptom scores after rhinovirus challenge (r=0.53 and r=0.64, respectively). Conclusions Rhinovirus infection by itself induces no IDO activity, but the reduced pulmonary IDO activity in patients with allergic asthma at baseline may underlie a reduced control of viral infections. Notably, the enhanced systemic catabolism of tryptophan in patients with allergic asthma was strongly related to the outcome of rhinovirus challenge in asthma and may serve as a prognostic factor. |
| تدمد: | 1468-3296 0040-6376 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc472609eca13fa50ec01a3c21ce25eaTest https://doi.org/10.1136/thoraxjnl-2013-203728Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dc472609eca13fa50ec01a3c21ce25ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14683296 00406376 |
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