Multi-species single-cell transcriptomic analysis of ocular compartment regulons
| العنوان: | Multi-species single-cell transcriptomic analysis of ocular compartment regulons |
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| المؤلفون: | Bar Makovoz, Pradeep Gautam, Katherine Anne Lau, Raymond C.B. Wong, Hu Li, Xinyi Su, Yuin-Han Loh, Hsin Yee Lee, Timothy A. Blenkinsop, Ying Chen, Woon-Khiong Chan, Kiyofumi Hamashima, Bhav Harshad Parikh, Yingying Zeng |
| المصدر: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Retinal Ganglion Cells, Cell type, genetic structures, Swine, Science, Cellular differentiation, General Physics and Astronomy, Retinal ganglion, Retina, Article, General Biochemistry, Genetics and Molecular Biology, Species Specificity, medicine, Animals, Humans, Compartment (development), Transcriptomics, Zebrafish, Aged, Aged, 80 and over, Adult stem cells, Multidisciplinary, biology, Sequence Analysis, RNA, Gene Expression Profiling, Cell Differentiation, General Chemistry, Middle Aged, biology.organism_classification, eye diseases, Gene regulation, Cell biology, Gene expression profiling, medicine.anatomical_structure, Eye disorder, sense organs, Single-Cell Analysis, Transcriptome |
| الوصف: | The retina is a widely profiled tissue in multiple species by single-cell RNA sequencing studies. However, integrative research of the retina across species is lacking. Here, we construct the first single-cell atlas of the human and porcine ocular compartments and study inter-species differences in the retina. In addition to that, we identify putative adult stem cells present in the iris tissue. We also create a disease map of genes involved in eye disorders across compartments of the eye. Furthermore, we probe the regulons of different cell populations, which include transcription factors and receptor-ligand interactions and reveal unique directional signalling between ocular cell types. In addition, we study conservation of regulons across vertebrates and zebrafish to identify common core factors. Here, we show perturbation of KLF7 gene expression during retinal ganglion cells differentiation and conclude that it plays a significant role in the maturation of retinal ganglion cells. A comprehensive analysis of the ocular networks among various tissues is necessary to understand eye physiology in health and disease. Here the authors present a multi-species single-cell transcriptomic atlas consisting of cells of the cornea, iris, ciliary body, neural retina, retinal pigmented epithelium, and choroid. |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9fa9f95cb4f031daa4b94f5089e3126Test https://doi.org/10.1038/s41467-021-25968-8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d9fa9f95cb4f031daa4b94f5089e3126 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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