An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells
| العنوان: | An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells |
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| المؤلفون: | Jean-Pierre Rona, Pascal Meimoun, Raffik Errakhi, François Bouteau, Carole Beaulieu, Karine Madiona, Parul Vatsa, David Reboutier, Arnaud Lehner, Mustapha Barakate, Joël Briand, David Wendehenne, Aurélien Dauphin |
| المساهمون: | Laboratoire d'Electrophysiologie des Membranes (LEM) EA 3514, Université Paris Diderot - Paris 7 (UPD7), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Université Cadi Ayyad [Marrakech] (UCA), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Sherbrooke [Sherbrooke], FLAveur, VIsion et Comportement du consommateur (FLAVIC), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Université de Bourgogne (UB), Université de Sherbrooke (UdeS) |
| المصدر: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2008, 59 (15), pp.4259-4270. ⟨10.1093/jxb/ern267⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | 0106 biological sciences, Programmed cell death, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Physiology, Cell, chemistry.chemical_element, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Plant Science, Biology, Calcium, 01 natural sciences, 03 medical and health sciences, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], medicine, Arabidopsis thaliana, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, 030304 developmental biology, 0303 health sciences, Voltage-dependent calcium channel, food and beverages, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Streptomyces scabies, biology.organism_classification, plant pathogen, Streptomyces, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, cell death, thaxtomin A, [CHIM.POLY]Chemical Sciences/Polymers, medicine.anatomical_structure, Biochemistry, chemistry, ion channel, Second messenger system, Signal transduction, 010606 plant biology & botany |
| الوصف: | International audience; The pathogenicity of various Streptomyces scabies isolates involved in potato scab disease was correlated with the production of thaxtomin A. Since calcium is known as an essential second messenger associated with pathogen-induced plant responses and cell death, it was investigated whether thaxtomin A could induce a Ca 2+ influx related to cell death and to other putative plant responses using Arabidopsis thaliana suspension cells, which is a convenient model to study plant–microbe interactions. A. thaliana cells were treated with micromolar concentrations of thaxto-min A. Cell death was quantified and ion flux variations were analysed from electrophysiological measurements with the apoaequorin Ca 2+ reporter protein and by external pH measurement. Involvement of anion and calcium channels in signal transduction leading to programmed cell death was determined by using specific inhibitors. These data suggest that this toxin induces a rapid Ca 2+ influx and cell death in A. thaliana cell suspensions. Moreover, these data provide strong evidence that the Ca 2+ influx induced by thaxtomin A is necessary to achieve this cell death and is a prerequisite to early thaxtomin A-induced responses: anion current increase, alkalization of the external medium, and the expression of PAL1 coding for a key enzyme of the phenylpropanoid pathway. |
| تدمد: | 1460-2431 0022-0957 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d903d2829b5fdf5b5363d5a746285c68Test https://doi.org/10.1093/jxb/ern267Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d903d2829b5fdf5b5363d5a746285c68 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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