Role of Tyrosine Kinase in Serotonin-Induced Constriction of the Basilar Artery In Vivo
| العنوان: | Role of Tyrosine Kinase in Serotonin-Induced Constriction of the Basilar Artery In Vivo |
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| المؤلفون: | Takanari Kitazono, Jiro Kitayama, Tetsuhiko Nagao, Setsuro Ibayashi, Tomoko Kagiyama, Masatoshi Fujishima |
| المصدر: | Stroke. 29:494-498 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Male, Serotonin, medicine.medical_specialty, Cerebral arteries, Protein tyrosine phosphatase, Muscle, Smooth, Vascular, Rats, Sprague-Dawley, chemistry.chemical_compound, Phenols, medicine.artery, Internal medicine, Nitriles, Basilar artery, medicine, Animals, Enzyme Inhibitors, Tyrosine, Sodium orthovanadate, Phorbol 12,13-Dibutyrate, Protein kinase C, Advanced and Specialized Nursing, Analysis of Variance, business.industry, Protein-Tyrosine Kinases, Tyrphostins, Genistein, Rats, Endocrinology, chemistry, Vasoconstriction, Basilar Artery, Neurology (clinical), Protein Tyrosine Phosphatases, Vanadates, medicine.symptom, Cardiology and Cardiovascular Medicine, business, Tyrosine kinase |
| الوصف: | Background and Purpose —Serotonin is one of the most potent constrictors of cerebral blood vessels and is implicated in several pathological conditions, including migraine and cerebral ischemia. Recent evidence has suggested that tyrosine kinase is involved in vasocontractile responses. The objective of this study was to test the hypothesis that activation of tyrosine kinase contributes to serotonin-induced constriction of the basilar artery in vivo. Methods —Using a cranial window in anesthetized Sprague-Dawley rats, we examined effects of inhibitors of tyrosine kinase and tyrosine phosphatase on constrictor responses of the basilar artery to serotonin in vivo. Results —Serotonin (10 −8 , 10 −7 , and 10 −6 mol/L) produced constriction of the basilar artery by 12±2%, 27±2%, and 37±3%, respectively. Genistein (3×10 −6 mol/L), an inhibitor of tyrosine kinase, did not affect baseline diameter of the basilar artery but attenuated serotonin-induced vasoconstriction ( P −5 mol/L), another inhibitor of tyrosine kinase, also attenuated serotonin-induced vasoconstriction, and tyrphostin 63, an inactive analogue of tyrphostin 47, did not affect the vasoconstriction. Sodium orthovanadate (10 −5 mol/L), an inhibitor of tyrosine phosphatase, enhanced serotonin-induced vasoconstriction. Phorbol 12,13-dibutyrate, a direct activator of protein kinase C, also caused constriction of the basilar artery, which was not affected by genistein or sodium orthovanadate. Conclusions —These results suggest that serotonin-induced constriction of the basilar artery is mediated, at least in part, by activation of tyrosine kinase in vivo. |
| تدمد: | 1524-4628 0039-2499 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d80040c3a0136eb2f0818deb7102d636Test https://doi.org/10.1161/01.str.29.2.494Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d80040c3a0136eb2f0818deb7102d636 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244628 00392499 |
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