Glioblastoma-derived Macrophage Colony-stimulating Factor (MCSF) Induces Microglial Release of Insulin-like Growth Factor-binding Protein 1 (IGFBP1) to Promote Angiogenesis
| العنوان: | Glioblastoma-derived Macrophage Colony-stimulating Factor (MCSF) Induces Microglial Release of Insulin-like Growth Factor-binding Protein 1 (IGFBP1) to Promote Angiogenesis |
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| المؤلفون: | Bangalore A. Chandramouli, Kumaravel Somasundaram, Serge Urbach, Vikas Patil, S D Shwetha, Kotha Sravani, Philippe Marin, Alangar S. Hegde, Mamatha B. Nijaguna, Vani Santosh, Arimappamagan Arivazhagan |
| المساهمون: | Indian Institute of Science [Bangalore] (IISc Bangalore), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), National Institute of Mental Health and Neurosciences [Bagalore, India] |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2015, 290 (38), pp.23401-23415. ⟨10.1074/jbc.M115.664037⟩ |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, genetic structures, Angiogenesis, medicine.medical_treatment, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, microglia, Biochemistry, SILAC, Phosphatidylinositol 3-Kinases, angiogenesis, cytokine, Tube formation, Microbiology & Cell Biology, Microglia, Neovascularization, Pathologic, Brain Neoplasms, Intracellular Signaling Peptides and Proteins, NF-kappa B, Protein-Tyrosine Kinases, 3. Good health, Cell biology, Neoplasm Proteins, Endothelial stem cell, medicine.anatomical_structure, Cytokine, endothelial cell, Female, psychological phenomena and processes, Signal Transduction, Macrophage colony-stimulating factor, Adult, MCSF, [SDV.CAN]Life Sciences [q-bio]/Cancer, macrophage, Biology, Glioma, Cell Line, Tumor, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, otorhinolaryngologic diseases, Human Umbilical Vein Endothelial Cells, Humans, Syk Kinase, Autocrine signalling, Molecular Biology, neoplasms, Macrophage Colony-Stimulating Factor, glioblastoma, Cell Biology, medicine.disease, nervous system diseases, Insulin-Like Growth Factor Binding Protein 1, IGFBP1 |
| الوصف: | International audience; Glioblastoma (grade IV glioma/GBM) is the most common primary adult malignant brain tumor with poor prognosis. To characterize molecular determinants of tumor-stroma interaction in GBM, we profiled 48 serum cytokines and identified macrophage colony-stimulating factor (MCSF) as one of the elevated cytokines in sera from GBM patients. Both MCSF transcript and protein were up-regulated in GBM tissue samples through a spleen tyrosine kinase (SYK)-dependent activation of the PI3K-NFκB pathway. Ectopic overexpression and silencing experiments revealed that glioma-secreted MCSF has no role in autocrine functions and M2 polarization of macrophages. In contrast, silencing expression of MCSF in glioma cells prevented tube formation of human umbilical vein endothelial cells elicited by the supernatant from monocytes/microglial cells treated with conditioned medium from glioma cells. Quantitative proteomics based on stable isotope labeling by amino acids in cell culture showed that glioma-derived MCSF induces changes in microglial secretome and identified insulin-like growth factor-binding protein 1 (IGFBP1) as one of the MCSF-regulated proteins secreted by microglia. Silencing IGFBP1 expression in microglial cells or its neutralization by an antibody reduced the ability of supernatants derived from microglial cells treated with glioma cell-conditioned medium to induce angiogenesis. In conclusion, this study shows up-regulation of MCSF in GBM via a SYK-PI3K-NFκB-dependent mechanism and identifies IGFBP1 released by microglial cells as a novel mediator of MCSF-induced angiogenesis, of potential interest for developing targeted therapy to prevent GBM progression. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9258 1083-351X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7f878d2a72ee5b4ff52a640a8cd8db5Test https://hal.umontpellier.fr/hal-02065415/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d7f878d2a72ee5b4ff52a640a8cd8db5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 1083351X |
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