POWERDRESS-mediated histone deacetylation is essential for thermomorphogenesis inArabidopsis thaliana
| العنوان: | POWERDRESS-mediated histone deacetylation is essential for thermomorphogenesis inArabidopsis thaliana |
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| المؤلفون: | Tasset, Celine, Singh Yadav, Avilash, Sureshkumar, Sridevi, Singh, Rupali, van der Woude, Lennard, Nekrasov, Maxim, Tremethick, David, van Zanten, Martijn, Balasubramanian, Sureshkumar, Sub Molecular Plant Physiology, Molecular Plant Physiology |
| المساهمون: | Sub Molecular Plant Physiology, Molecular Plant Physiology |
| المصدر: | PLoS Genetics, Vol 14, Iss 3, p e1007280 (2018) PLoS Genetics, 14(3). Public Library of Science PLoS Genetics |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, 0106 biological sciences, Cancer Research, Fruit and Seed Anatomy, Arabidopsis, Histones/metabolism, Gene Expression, Plant Science, Biochemistry, 01 natural sciences, Histones, Histone methylation, Morphogenesis, Arabidopsis thaliana, Histone code, Genetics (clinical), Plant Growth and Development, Regulation of gene expression, 2. Zero hunger, Genetics, 0303 health sciences, Chromosome Biology, Plant Anatomy, Temperature, Eukaryota, food and beverages, Acetylation, Genomics, Plants, Hypocotyl, Chromatin, Nucleosomes, Cell biology, Arabidopsis Proteins/genetics, Histone, Experimental Organism Systems, Histone methyltransferase, Embryogenesis, Epigenetics, Transcriptome Analysis, Research Article, lcsh:QH426-470, Arabidopsis Thaliana, Transcription Factors/genetics, Plant Development, Brassica, SAP30, Biology, Research and Analysis Methods, Histone Deacetylases, Chromatin remodeling, 03 medical and health sciences, Model Organisms, Histone H1, Plant and Algal Models, DNA-binding proteins, Histone H2A, Nucleosome, Gene Regulation, Arabidopsis/genetics, Molecular Biology, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Biology and life sciences, Plant Embryo Anatomy, Arabidopsis Proteins, HDAC9, Organisms, Proteins, Computational Biology, Cell Biology, Genome Analysis, biology.organism_classification, lcsh:Genetics, 030104 developmental biology, Mutation, biology.protein, Histone Deacetylases/metabolism, Plant Embryogenesis, Developmental Biology, Transcription Factors, 010606 plant biology & botany |
| الوصف: | Ambient temperature affects plant growth and even minor changes can substantially impact crop yields. The underlying mechanisms of temperature perception and response are just beginning to emerge. Chromatin remodeling, via the eviction of the histone variant H2A.Z containing nucleosomes, is a critical component of thermal response in plants. However, the role of histone modifications remains unknown. Here, through a forward genetic screen, we identify POWERDRESS (PWR), a SANT-domain containing protein known to interact with HISTONE DEACETYLASE 9 (HDA9), as a novel factor required for thermomorphogenesis in Arabidopsis thaliana. We show that mutations in PWR impede thermomorphogenesis, exemplified by attenuated warm temperature-induced hypocotyl/petiole elongation and early flowering. We show that inhibitors of histone deacetylases diminish temperature-induced hypocotyl elongation, which demonstrates a requirement for histone deacetylation in thermomorphogenesis. We also show that elevated temperature is associated with deacetylation of H3K9 at the +1 nucleosomes of PHYTOCHROME INTERACTING FACTOR4 (PIF4) and YUCCA8 (YUC8), and that PWR is required for this response. There is global misregulation of genes in pwr mutants at elevated temperatures. Meta-analysis revealed that genes that are misregulated in pwr mutants display a significant overlap with genes that are H2A.Z-enriched in their gene bodies, and with genes that are differentially expressed in mutants of the components of the SWR1 complex that deposits H2A.Z. Our findings thus uncover a role for PWR in facilitating thermomorphogenesis and suggest a potential link between histone deacetylation and H2A.Z nucleosome dynamics in plants. Author summary Plant growth and development is influenced by a variety of external environmental cues. Ambient temperature affects almost all stages of plant development but the underlying molecular mechanisms remain largely unknown. In this paper, the authors show that histone deacetylation, an important chromatin remodeling processes, is essential for eliciting warm temperature-induced growth responses in plants; a process called thermomorphogenesis. The authors identify POWERDRESS, a protein known to interact with HISTONE DEACETYLASE 9, as a novel player essential for thermomorphogenesis in Arabidopsis. Another chromatin remodeling mechanism that is known to play a role in thermal response is the eviction of histone variant H2A.Z containing nucleosomes. Through transcriptome studies and meta-analysis, the authors demonstrate statistical associations between gene regulations conferred through PWR-mediated histone H3 deacetylation and those conferred by histone H2A.Z eviction/incorporation dynamics. This study identifies a novel gene that is essential for thermomorphogenesis and points to a possible link between two seemingly distinct chromatin-remodeling processes in regulating gene expression in plants. |
| وصف الملف: | image/pdf |
| تدمد: | 1553-7390 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d57a60975b88b4bf219bb7925e75a4bbTest https://doi.org/10.1101/186916Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d57a60975b88b4bf219bb7925e75a4bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15537390 |
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