Development of a High-Resolution Single-Nucleotide Polymorphism Strain-Typing Assay Using Whole Genome-Based Analyses for the Lactobacillus acidophilus Probiotic Strain
| العنوان: | Development of a High-Resolution Single-Nucleotide Polymorphism Strain-Typing Assay Using Whole Genome-Based Analyses for the Lactobacillus acidophilus Probiotic Strain |
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| المؤلفون: | Yu-Chun Lin, Shih-Hau Chiu, Chih-Chieh Chen, Jong-Shian Liou, Koichi Watanabe, Jin-Seng Lin, Chien-Hsun Huang, Lina Huang |
| المصدر: | Microorganisms, Vol 8, Iss 1445, p 1445 (2020) Microorganisms Volume 8 Issue 9 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), Lactobacillus acidophilus, Single-nucleotide polymorphism, core genome MLST, Biology, Microbiology, Genome, law.invention, 03 medical and health sciences, Probiotic, law, Virology, Genotype, Multiplex, lcsh:QH301-705.5, monophyletic bacterium, Polymerase chain reaction, 030304 developmental biology, Genetics, 0303 health sciences, 030306 microbiology, Strain (biology), food and beverages, lcsh:Biology (General), whole genome sequences, Strain-Specific Identification |
| الوصف: | Lactobacillus acidophilus is one of the most commonly used industrial products worldwide. Since its probiotic efficacy is strain-specific, the identification of probiotics at both the species and strain levels is necessary. However, neither phenotypic nor conventional genotypic methods have enabled the effective differentiation of L. acidophilus strains. In this study, a whole-genome sequence-based analysis was carried out to establish high-resolution strain typing of 41 L. acidophilus strains (including commercial isolates and reference strains) using the cano-wgMLST_BacCompare analytics platform consequently, a strain-specific discrimination method for the probiotic strain LA1063 was developed. Using a core-genome multilocus sequence-typing (cgMLST) scheme based on 1390 highly conserved genes, 41 strains could be assigned to 34 sequence types. Subsequently, we screened a set of 92 loci with a discriminatory power equal to that of the 1390 loci cgMLST scheme. A strain-specific polymerase chain reaction combined with a multiplex minisequencing method was developed based on four (phoU, secY, tilS, and uvrA_1) out of 21 loci, which could be discriminated between LA1063 and other L. acidophilus strains using the cgMLST data. We confirmed that the strain-specific single-nucleotide polymorphisms method could be used to quickly and accurately identify the L. acidophilus probiotic strain LA1063 in commercial products. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-2607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d42b1f58f21ba52b9f9a786f1838a511Test https://doi.org/10.3390/microorganisms8091445Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d42b1f58f21ba52b9f9a786f1838a511 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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