Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells
| العنوان: | Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells |
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| المؤلفون: | Joanna Rzemieniec, Wojciech Krzeptowski, Władysław Lasoń, Agnieszka Wnuk, Małgorzata Kajta |
| المصدر: | Molecular Neurobiology |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Time Factors, Retinoid X receptors, Apoptosis, Neocortex, Benzoates, Epigenesis, Genetic, primary neuronal cell cultures, Mice, Benzophenone-3, Phagosomes, RNA, Small Interfering, Cells, Cultured, Histone Acetyltransferases, Neurons, education.field_of_study, Caspase 3, retinoid X receptors, Primary neuronal cell cultures, Apoptotic body, Cell biology, Histone, Neurology, DNA methylation, Microtubule-Associated Proteins, Signal Transduction, autophagy, Population, RXR, Neuroscience (miscellaneous), Biology, Retinoid X receptor, BP-3, benzophenone-3, Article, Histone Deacetylases, Benzophenones, 03 medical and health sciences, Cellular and Molecular Neuroscience, Downregulation and upregulation, Autophagy, Animals, RNA, Messenger, Epigenetics, education, L-Lactate Dehydrogenase, Staining and Labeling, Gene Expression Profiling, Biphenyl Compounds, DNA Methylation, 030104 developmental biology, biology.protein |
| الوصف: | Benzophenone-3 (BP-3) is the most widely used compound among UV filters for the prevention of photodegradation. Population studies have demonstrated that it penetrates through the skin and crosses the blood-brain barrier. However, little is known about the impact of BP-3 on the nervous system and its possible adverse effects on the developing brain. We demonstrated that the neurotoxic effects of BP-3 were accompanied by the induction of apoptosis, as evidenced by apoptosis-related caspase-3 activation and apoptotic body formation as well as the inhibition of autophagy, as determined by the downregulation of autophagy-related genes, decreased autophagosome formation, and reduced LC3B-to-LC3A ratio. In this study, we showed for the first time that the BP-3-induced apoptosis of neuronal cells is mediated via the stimulation of RXRα signaling and the attenuation of RXRβ/RXRγ signaling, as demonstrated using selective antagonist and specific siRNAs as well as by measuring the mRNA and protein expression levels of the receptors. This study also demonstrated that environmentally relevant concentrations of BP-3 were able to inhibit autophagy and disrupt the epigenetic status of neuronal cells, as evidenced by the inhibition of global DNA methylation as well as the reduction of histone deacetylases and histone acetyl transferases activity, which may increase the risks of neurodevelopmental abnormalities and/or neural degenerations. |
| تدمد: | 1559-1182 0893-7648 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3809e3619588e574d3d0acaf74fe9deTest https://doi.org/10.1007/s12035-017-0704-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d3809e3619588e574d3d0acaf74fe9de |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15591182 08937648 |
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