Circular RNA circ_0000712 regulates high glucose-induced apoptosis, inflammation, oxidative stress, and fibrosis in (DN) by targeting the miR-879-5p/SOX6 axis
| العنوان: | Circular RNA circ_0000712 regulates high glucose-induced apoptosis, inflammation, oxidative stress, and fibrosis in (DN) by targeting the miR-879-5p/SOX6 axis |
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| المؤلفون: | Li Zhao, Tao Yang, Kun Yang, Yan Zeng, Zhengdong Li, Ren Zhang, Hualing Ruan, Huaqian Chen |
| المصدر: | Endocrine Journal. 68:1155-1164 |
| بيانات النشر: | Japan Endocrine Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Collagen Type IV, Endocrinology, Diabetes and Metabolism, Interleukin-1beta, Apoptosis, medicine.disease_cause, Collagen Type I, Superoxide dismutase, Mice, chemistry.chemical_compound, Endocrinology, Downregulation and upregulation, Lactate dehydrogenase, medicine, Animals, Diabetic Nephropathies, Transcription factor, bcl-2-Associated X Protein, Inflammation, chemistry.chemical_classification, Reactive oxygen species, L-Lactate Dehydrogenase, biology, Interleukin-6, Superoxide Dismutase, Tumor Necrosis Factor-alpha, Chemistry, RNA, Circular, Molecular biology, Fibronectins, MicroRNAs, Oxidative Stress, Real-time polymerase chain reaction, Proto-Oncogene Proteins c-bcl-2, Hyperglycemia, Mesangial Cells, biology.protein, Reactive Oxygen Species, SOXD Transcription Factors, Oxidative stress |
| الوصف: | Diabetic nephropathy (DN), a frequent diabetes complication, has complex pathogenesis. Circular RNAs (circRNAs) circ_0000712 has been reported to be upregulated in kidney tissues and high glucose (HG)-inducted Mesangial cells (MCs). This study is designed to explore the role and mechanism of circ_0000712 in the HG-inducted MCs injury in DN. Circ_0000712, microRNA-879-5p (miR-879-5p), and SRY-Box Transcription Factor 6 (SOX6) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell apoptosis was examined by flow cytometry assay. Protein levels of B-cell lymphoma-2 (Bcl-2), Bcl-2 related X protein (Bax), fibronectin (FN), collagen type I (Col. I), collagen type IV (Col. IV), and SOX6 were assessed by western blot assay. Levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) generation, Lactate Dehydrogenase (LDH) activity, and Superoxide Dismutase (SOD) activity were detected by the corresponding kits. The binding relationship between miR-879-5p and circ_0000712 or SOX6 was predicted by starBase and Targetscan, and then verified by a dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. Circ_0000712 and SOX6 were highly expressed, and miR-879-5p was decreased in db/db DN mice and HG-inducted SV40-MES13 cells. Furthermore, circ_0000712 deficiency repressed HG-caused apoptosis, inflammation, oxidative stress, and fibrosis in SV40-MES13 cells. Mechanically, circ_0000712 could regulate SOX6 expression by sponging miR-879-5p. Circ_0000712 knockdown could hinder HG-inducted SV40-MES13 cell injury through targeting the miR-879-5p/SOX6 axis, implying a possible circRNA-targeted therapy for DN. |
| تدمد: | 1348-4540 0918-8959 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d349accd8d8a3bc1ce4cd068368ca634Test https://doi.org/10.1507/endocrj.ej20-0739Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d349accd8d8a3bc1ce4cd068368ca634 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13484540 09188959 |
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