Metabolic Engineering of Enterobacter aerogenes for Improved 2,3-Butanediol Production by Manipulating NADH Levels and Overexpressing the Small RNA RyhB
| العنوان: | Metabolic Engineering of Enterobacter aerogenes for Improved 2,3-Butanediol Production by Manipulating NADH Levels and Overexpressing the Small RNA RyhB |
|---|---|
| المؤلفون: | Ke Jiang, Ping Lu, Yefei Liu, Yudong Xu, Haoning Yang, Qi Shen, Jiayao Yang, Yan Wu, Fangxu Xu, Wanying Chu, Hongxin Zhao |
| المصدر: | Frontiers in Microbiology, Vol 12 (2021) Frontiers in Microbiology |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), biology, Chemistry, NADH dehydrogenase, lactate dehydrogenase, Enterobacter aerogenes, 2,3-butanediol, biology.organism_classification, Microbiology, RyhB, QR1-502, Metabolic engineering, chemistry.chemical_compound, Biochemistry, small RNA RyhB, Lactate dehydrogenase, Metabolic flux analysis, biology.protein, NAD+ kinase, Flux (metabolism), Original Research |
| الوصف: | Biotechnological production of 2,3-butanediol (2,3-BD), a versatile platform bio-chemical and a potential biofuel, is limited due to by-product toxicity. In this study, we aimed to redirect the metabolic flux toward 2,3-BD in Enterobacter aerogenes (E. aerogenes) by increasing the intracellular NADH pool. Increasing the NADH/NAD+ ratio by knocking out the NADH dehydrogenase genes (nuoC/nuoD) enhanced 2,3-BD production by up to 67% compared with wild-type E. aerogenes. When lactate dehydrogenase (ldh) was knocked out, the yield of 2,3-BD was increased by 71.2% compared to the wild type. Metabolic flux analysis revealed that upregulated expression of the sRNA RyhB led to a noteworthy shift in metabolism. The 2,3-BD titer of the best mutant Ea-2 was almost seven times higher than that of the parent strain in a 5-L fermenter. In this study, an effective metabolic engineering strategy for improved 2,3-BD production was implemented by increasing the NADH/NAD+ ratio and blocking competing pathways. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2efe973760d5a6e851b43d3f55c9f54Test https://www.frontiersin.org/articles/10.3389/fmicb.2021.754306/fullTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d2efe973760d5a6e851b43d3f55c9f54 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |