Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NFκB in Jurkat cells
| العنوان: | Melibiosamine, a novel oligosaccharide, suppresses mitogen-induced IL-2 production via inactivation of NFAT and NFκB in Jurkat cells |
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| المؤلفون: | Daichi Aoki, Kyoichi Iizumi, Osamu Hosomi, Kaoru Uchida, Makoto Ogata, Shuri Akiyama, Susumu Tanaka, Yuzuru Kubohara, Kazuki Fujita |
| المصدر: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 19, Iss, Pp-(2019) |
| بيانات النشر: | Elsevier BV, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | PMA, phorbol 12-myristate 13-acetate, 0301 basic medicine, Interleukin 2, CIIP, ConA-induced IL-2 production, NFκB, nuclear factor kappa-light-chain-enhancer of activated B cells, Biophysics, ConA, concanavalin A, PIIP, PMA/IM-induced IL-2 production, AP-1, activator protein 1, Biochemistry, Jurkat cells, lcsh:Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Immune system, IL-2, interleukin-2, medicine, lcsh:QD415-436, Viability assay, lcsh:QH301-705.5, Transcription factor, IM, ionomycin, Glucosamine, GlcNH2, glucosamine, biology, Immunosuppressive drug, Chemistry, NFAT, Melibiosamine, Cell biology, NFAT, nuclear factor of activated T-cells, 030104 developmental biology, lcsh:Biology (General), Concanavalin A, Cell culture, 030220 oncology & carcinogenesis, biology.protein, Jurkat cell, CsA, cyclosporine A, Research Article, MelNH2, melibiosamine, medicine.drug |
| الوصف: | d-Glucosamine (GlcNH2) and several of its derivatives are known to possess immunosuppressive activities in various immune cell lines. The novel GlcNH2-containing oligosaccharide Galα1-6GlcNH2 (designated melibiosamine; MelNH2) is expected to be immunosuppressive also. In Jurkat cells (immortalized human T lymphocytes), interleukin 2 (IL-2) production (an index of the T-cell immune response) can be induced by stimulation with a mitogen, such as concanavalin A. Here, we compared the effects of GlcNH2 and MelNH2 on concanavalin A-induced IL-2 production (CIIP) in Jurkat cells and found that GlcNH2 and MelNH2 at millimolar levels both significantly suppressed CIIP without affecting cell viability. When we examined the effects of GlcNH2 and MelNH2 on the activation of the three transcription factors required for CIIP—NFAT (nuclear factor of activated T-cells), NFκB (nuclear factor kappa-light-chain-enhancer of activated B cells), and AP-1 (activator protein 1)—we found that GlcNH2 and MelNH2 both suppressed CIIP by inhibiting the activation of NFAT and NFκB, but, unlike GlcNH2, MelNH2 also promoted the activation of AP-1. These results suggest that MelNH2 may be a potentially useful lead compound for development as an immunosuppressive or anti-inflammatory drug. Highlights • Immunosuppressive effects of MelNH2 (Galα1-6GlcNH2) were examined in Jurkat cells. • Concanavalin A induces IL-2 production in Jurkat cells. • MelNH2 at millimolar levels dose-dependently suppressed ConA-induced IL-2 production. • MelNH2 inhibited the activation of NFAT and NFκB, which control IL-2 expression. |
| تدمد: | 2405-5808 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1d464936fd14de259a844061a4ec2bdTest https://doi.org/10.1016/j.bbrep.2019.100658Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1d464936fd14de259a844061a4ec2bd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24055808 |
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