Cytokine Signature in Schnitzler Syndrome: Proinflammatory Cytokine Production Associated to Th Suppression
| العنوان: | Cytokine Signature in Schnitzler Syndrome: Proinflammatory Cytokine Production Associated to Th Suppression |
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| المؤلفون: | Marie Masson Regnault, Eric Frouin, Isabelle Jéru, Adriana Delwail, Sandrine Charreau, Sébastien Barbarot, Antoine Néel, Agathe Masseau, Xavier Puéchal, Xavier Kyndt, Stephane Gayet, François Lifermann, Bouchra Asli, Xavier Balguerie, Claire Blanchard-Delaunay, François Aubin, Rita Rizzi, Franco Rongioletti, Thierry Boyé, Laurence Gusdorf, Didier Bessis, Franck Morel, Ewa Hainaut, Dan Lipsker, Jean-Claude Lecron |
| المساهمون: | Masson Regnault, M., Frouin, E., Jeru, I., Delwail, A., Charreau, S., Barbarot, S., Neel, A., Masseau, A., Puechal, X., Kyndt, X., Gayet, S., Lifermann, F., Asli, B., Balguerie, X., Blanchard-Delaunay, C., Aubin, F., Rizzi, R., Rongioletti, F., Boye, T., Gusdorf, L., Bessis, D., Morel, F., Hainaut, E., Lipsker, D., Lecron, J. -C. |
| المصدر: | Frontiers in Immunology Frontiers in Immunology, Vol 11 (2020) |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | lcsh:Immunologic diseases. Allergy, 0301 basic medicine, Adult, Lipopolysaccharides, Male, PBMC (peripheral blood mononuclear cells), medicine.medical_treatment, Immunology, Inflammation, Peripheral blood mononuclear cell, Proinflammatory cytokine, 03 medical and health sciences, 0302 clinical medicine, Schnitzler syndrome, inflammasome, Gammopathy, medicine, Immunology and Allergy, Humans, Cells, Cultured, Original Research, Aged, Aged, 80 and over, business.industry, IL-1, IL-1 antagonist, Interleukin, T-Lymphocytes, Helper-Inducer, Middle Aged, medicine.disease, cytokines, Interleukin 1 Receptor Antagonist Protein, 030104 developmental biology, Cytokine, 030228 respiratory system, Antirheumatic Agents, ex vivo, Tumor necrosis factor alpha, Female, medicine.symptom, lcsh:RC581-607, business |
| الوصف: | BackgroundSchnitzler syndrome (SchS) is a rare autoinflammatory disease characterized by urticarial exanthema, bone and joint alterations, fever and monoclonal IgM gammopathy. Overactivation of the interleukin(IL)-1 system is reported, even though the exact pathophysiological pathways remain unknown.ObjectiveTo determine ex vivo cytokine profiles of Peripheral Blood Mononuclear Cells (PBMCs) from SchS patients prior to treatment and after initiation of anti-IL-1 therapy (anakinra). The sera cytokine profile was studied in parallel.MethodsWe collected blood samples from thirty-six untreated or treated SchS. PBMCs were cultured with and without LPS or anti-CD3/CD28. Cytokine levels were evaluated in serum and cell culture supernatants using Luminex technology.ResultsSpontaneous TNFα, IL-6, IL-1β, IL-1α, and IL-1RA release by PBMCs of SchS patients were higher than in controls. LPS-stimulation further induced the secretion of these cytokines. In contrast, after T-cell stimulation, TNFα, IL-10, IFNγ, IL-17A, and IL-4 production decreased in SchS patients compared to healthy controls, but less in treated patients. Whereas IL-1β serum level was not detected in most sera, IL-6, IL-10, and TNFα serum levels were higher in patients with SchS and IFNγ and IL-4 levels were lower. Of note, IL-6 decreased after treatment in SchS (p = 0.04).ConclusionOur data strengthen the hypothesis of myeloid inflammation in SchS, mediated in particular by IL-1β, TNFα, and IL-6, associated with overproduction of the inhibitors IL-1RA and IL-10. In contrast, we observed a loss of Th1, Th2, and Th17 cell functionalities that tends to be reversed by anakinra. |
| اللغة: | English |
| تدمد: | 1664-3224 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1bf8db0a701bb7d4c3700a84fa211edTest http://europepmc.org/articles/PMC7726442Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1bf8db0a701bb7d4c3700a84fa211ed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16643224 |
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