Detection of human papillomavirus distinguishes second primary tumors from lung metastases in patients with squamous cell carcinoma of the cervix
| العنوان: | Detection of human papillomavirus distinguishes second primary tumors from lung metastases in patients with squamous cell carcinoma of the cervix |
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| المؤلفون: | Changfei Qin, Lihong Zhang, Jia-Bin Lu, Suxia Lin, Fang Wang, Xiaoxuan Li, Shengbing Zang, Jie-Tian Jin, Qunxi Chen, Xinke Zhang, Taoli Wang |
| المصدر: | Thoracic Cancer Thoracic Cancer, Vol 11, Iss 8, Pp 2297-2305 (2020) |
| بيانات النشر: | John Wiley & Sons Australia, Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Pulmonary and Respiratory Medicine, squamous cell carcinoma, Male, Pathology, medicine.medical_specialty, Human papillomavirus, Lung Neoplasms, Papillomavirus E7 Proteins, Uterine Cervical Neoplasms, Alphapapillomavirus, lcsh:RC254-282, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Medicine, Humans, Basal cell, In patient, Neoplasm Metastasis, Cervix, second primary tumor, Aged, Tissue microarray, Lung, RNAscope assay, business.industry, lung metastasis, General Medicine, Second primary cancer, Original Articles, respiratory system, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Immunohistochemistry, Original Article, Female, business |
| الوصف: | Background In patients with squamous cell carcinoma of the cervix (SCCC), a squamous cell carcinoma (SqCC) in the lung represents either a second primary tumor or metastasis. This distinction between second primary tumors and lung metastases in patients with SCCC significantly influences patient prognosis and therapy. Here, we aimed to differentiate second primary tumors from lung metastases in patients with SCCC by exploring the HPV status in SqCCs involving the lung within a large cohort. Methods P16 expression was assessed using immunohistochemistry on tissue microarrays including a total of 415 primary lung SqCCs and 21 lung SqCCs with prior SCCC. Following this, we performed HPV DNA typing and the sensitive RNAscope in situ method to screen all the cases for HPV E6/E7 expression, which is a more reliable indicator of transcriptively active HPV in tumor cells. Results The p16 positive expression rate was 13.7% (57/415) in primary lung SqCCs, but HPV DNA was not detected in any of the 57 primary lung SqCC cases that positively expressed p16. In contrast, HPV DNA was detected in all cases (21/21) with prior SCCC. Consistently, all 21 lung SqCCs with prior SCCC (21/21) showed extensive HPV16 E6/E7 expression. In striking contrast, none of the primary lung SqCCs (0/415) had a detectable RNAscope signal. Conclusions HPV does not seem to play a role in the development of primary lung SqCCs. HPV detection may be helpful in distinguishing second primary tumors from lung metastases in patients with SCCC. HPV does not seem to play a role in the development of primary lung SqCCs. HPV detection may be helpful in distinguishing second primary tumors from lung metastases in patients with SCCC. |
| اللغة: | English |
| تدمد: | 1759-7714 1759-7706 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0019baf85c0997f59c42d748c14c00bTest http://europepmc.org/articles/PMC7396378Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d0019baf85c0997f59c42d748c14c00b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17597714 17597706 |
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