Aberrant populations of circulating T follicular helper cells and regulatory B cells underlying idiopathic pulmonary fibrosis
| العنوان: | Aberrant populations of circulating T follicular helper cells and regulatory B cells underlying idiopathic pulmonary fibrosis |
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| المؤلفون: | Hirotaka Nishikiori, Shingo Ichimiya, Hirofumi Chiba, Katsunori Shigehara, Ryuta Kamekura, Hiroki Takahashi, Yuichiro Asai, Satsuki Miyajima, Hayato Yabe, Shun Kondo |
| المصدر: | Respiratory Research Respiratory Research, Vol 20, Iss 1, Pp 1-9 (2019) |
| بيانات النشر: | BioMed Central, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Idiopathic pulmonary fibrosis (IPF), T follicular helper cell (Tfh cell), autoimmunity immunity, Programmed Cell Death 1 Receptor, Autoimmunity, Lymphocyte Activation, CXCR5, regulatory B cell (Breg cell), Idiopathic pulmonary fibrosis, 0302 clinical medicine, Diffusing capacity, Medicine, CXCR5 (C-X-C motif chemokine receptor 5), Lung, Aged, 80 and over, B-Lymphocytes, Regulatory, biology, medicine.diagnostic_test, T-Lymphocytes, Helper-Inducer, Middle Aged, Phenotype, Female, Antibody, ICOS (inducible co-stimulatory molecule), Receptors, CXCR5, Regulatory B cells, Peripheral blood mononuclear cell, PD-1 (programmed death 1), Flow cytometry, Inducible T-Cell Co-Stimulator Protein, 03 medical and health sciences, Humans, CXCL13, Aged, Cell Proliferation, lcsh:RC705-779, business.industry, Research, lcsh:Diseases of the respiratory system, medicine.disease, Idiopathic Pulmonary Fibrosis, CD4 Lymphocyte Count, Immunity, Humoral, 030104 developmental biology, Case-Control Studies, Immunology, biology.protein, Pulmonary Diffusing Capacity, business, Biomarkers, 030215 immunology |
| الوصف: | Background T follicular helper (Tfh) cells have been identified as a new category of helper T cells, which express CXCR5 on their surface and induce the production of antigen-specific antibodies. Many investigations have found morbid proliferation and/or activation of Tfh cells in systemic autoimmune and allergic diseases. It is also known that Tfh cells are regulated by regulatory B (Breg) cells in the deteriorating such diseases. Recently, CXCL13, a ligand of CXCR5, has been reported to increase in the peripheral blood and lungs of patients with idiopathic pulmonary fibrosis (IPF). This study aimed to investigate the involvement of Tfh cells and Breg cells in IPF. Methods Peripheral blood samples were obtained from 18 patients with IPF. We isolated heparinized peripheral blood mononuclear cells and investigated the proportions of Breg cells, Tfh cells, PD-1+ICOS+ Tfh cells (activated form of Tfh cells), and the Tfh-cell subsets by flow cytometry. These cell profiles were compared with those of 21 healthy controls. Furthermore, we investigated the correlations between profiles of lymphocytes and lung physiology. Results The median proportions of Tfh cells per total CD4+ T cells and of PD-1+ICOS+ proportion of Tfh cells per total Tfh cells was significantly more in the IPF patients (20.4 and 5.2%, respectively) compared with healthy controls (15.4 and 2.1%, respectively; p = 0.042 and p = 0.004, respectively). The proportion of Tfh2 cells per total Tfh cells was significantly higher and the proportion of Tfh17 was smaller in the IPF patients than healthy controls. The percentage of Breg cells to total B cells was significantly decreased in the IPF patients (median, 8.5%) compared with that in the controls (median, 19.7%; p r = 0.583, p = 0.018). Conclusion Proliferation and activation of Tfh cells and a decrease in Breg cells were observed in the peripheral blood of patients with IPF. The profile of the Tfh-cell subset also changed. Specific humoral immunity aberration would likely underlie complicated pathophysiology of IPF. |
| اللغة: | English |
| تدمد: | 1465-993X 1465-9921 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf2c2f9159e4519007ddd9060273c10bTest http://europepmc.org/articles/PMC6836348Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cf2c2f9159e4519007ddd9060273c10b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1465993X 14659921 |
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