AKT2 is involved in the IL-17A-mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3-E1 cells
| العنوان: | AKT2 is involved in the IL-17A-mediated promotion of differentiation and calcification of murine preosteoblastic MC3T3-E1 cells |
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| المؤلفون: | Pei‑Hui Ding, Lili Chen, Xiao‑Tao Chen, Jing‑Yi Tan, Li‑Hong Lei, Yan‑Min Wu |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, RAC-β serine/threonine protein kinase, Cancer Research, Cell, Osteocalcin, Core Binding Factor Alpha 1 Subunit, Biology, Biochemistry, interleukin-17A, 03 medical and health sciences, Mice, Phosphatidylinositol 3-Kinases, 0302 clinical medicine, Calcification, Physiologic, Genetics, medicine, Animals, Humans, Periodontitis, Molecular Biology, PI3K/AKT/mTOR pathway, Cell Proliferation, Osteoblasts, Cell growth, osteoblast calcification, Interleukin-17, Osteoblast, Cell Differentiation, 030206 dentistry, Articles, Cell cycle, medicine.disease, Alkaline Phosphatase, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Oncology, Gene Expression Regulation, osteoblast differentiation, Gene Knockdown Techniques, Molecular Medicine, Alkaline phosphatase, Signal transduction, Proto-Oncogene Proteins c-akt, Calcification, Signal Transduction |
| الوصف: | Interleukin (IL)‑17A exhibits pleiotropic biological activities and serves a role in the progression of periodontitis. However, data describing the association between IL‑17 and osteogenesis are not conclusive. It was previously demonstrated that RAC‑β serine/threonine protein kinase (AKT2)‑specific knockdown in MC3T3‑E1 cells weakened osteogenic effects. The role of AKT2 in the regulation of IL‑17A for osteoblast differentiation and calcification remains unclear. The MTT method was adopted in the present study to assess cell proliferation; cell cycle distribution was analyzed by flow cytometry. Following osteogenic induction treatment, the involvement of phosphatidylinositol 3‑kinase (PI3K) and phosphorylated‑PI3K was evaluated by western blotting. The effects of IL‑17A on osteogenesis‑associated markers, including Runt‑related transcription factor 2 (Runx‑2), alkaline phosphatase (ALP) and osteocalcin (OCN) were evaluated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. An ALP activity assay and Alizarin Red S staining were used to assess the differentiation and calcification functions. AKT2 knockdown inhibited MC3T3‑E1 cell proliferation, inducing significantly increased G0/G1 cell counts, and reduced S and G2/M cell numbers. IL‑17A exerted no significant effects. The protein levels of p‑PI3K, gene expression levels of IL‑17A, Runx‑2, ALP and OCN, and relative ALP activity and calcification areas were increased in the induction group, and these effects were markedly promoted by treatment with IL‑17A. AKT2 knockdown in MC3T3‑E1 cells resulted in reduced IL‑17A‑induced differentiation and calcification, although it was not completely inhibited. The results of the present study suggested that AKT2 signaling was required for MC3T3‑E1 cell proliferation. IL‑17A promoted osteoblast differentiation and calcification in a partly AKT2‑dependent manner in MC3T3‑E1 cells in vitro, possibly reflecting compensation by other signaling pathways. The results of the present study may offer novel perspectives to guide the clinical strategy for the prevention and treatment of periodontitis. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce984650a96b5f1c0414ed4b09b10f61Test http://europepmc.org/articles/PMC5865781Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ce984650a96b5f1c0414ed4b09b10f61 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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