The continuing evolution of the Langendorff and ejecting murine heart: new advances in cardiac phenotyping
| العنوان: | The continuing evolution of the Langendorff and ejecting murine heart: new advances in cardiac phenotyping |
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| المؤلفون: | Chee Chew Lim, Bruno K. Podesser, Ronglih Liao |
| المصدر: | American Journal of Physiology-Heart and Circulatory Physiology. 303:H156-H167 |
| بيانات النشر: | American Physiological Society, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Isolated Heart Preparation, Langendorff heart, Physiology, Reviews, Disease, Biology, History, 21st Century, Mice, Vascular reactivity, Physiology (medical), medicine, Animals, Humans, Cardiac electrophysiology, Heart, History, 19th Century, Stroke Volume, Isolated heart, History, 20th Century, medicine.disease, Myocardial Contraction, Mice, Mutant Strains, Cardiovascular physiology, Perfusion, Heart failure, Tissue and Organ Harvesting, Cardiac Electrophysiology, Anura, Cardiology and Cardiovascular Medicine, Neuroscience |
| الوصف: | The isolated retrograde-perfused Langendorff heart and the isolated ejecting heart have, over many decades, resulted in fundamental discoveries that form the underpinnings of our current understanding of the biology and physiology of the heart. These two experimental methodologies have proven invaluable in studying pharmacological effects on myocardial function, metabolism, and vascular reactivity and in the investigation of clinically relevant disease states such as ischemia-reperfusion injury, diabetes, obesity, and heart failure. With the advent of the genomics era, the isolated mouse heart preparation has gained prominence as an ex vivo research tool for investigators studying the impact of gene modification in the intact heart. This review summarizes the historical development of the isolated heart and provides a practical guide for the establishment of the Langendorff and ejecting heart preparations with a particular emphasis on the murine heart. In addition, current applications and novel methods of recording cardiovascular parameters in the isolated heart preparation will be discussed. With continued advances in methodological recordings, the isolated mouse heart preparation will remain physiologically relevant for the foreseeable future, serving as an integral bridge between in vitro assays and in vivo approaches. |
| تدمد: | 1522-1539 0363-6135 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccd04c0d9da035a45599355a55d7165eTest https://doi.org/10.1152/ajpheart.00333.2012Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ccd04c0d9da035a45599355a55d7165e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221539 03636135 |
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