Effect of hypoxia on the expression of CCAAT/enhancer-binding protein β and receptor activator of nuclear factor kappa-B ligand in periodontal ligament cells
| العنوان: | Effect of hypoxia on the expression of CCAAT/enhancer-binding protein β and receptor activator of nuclear factor kappa-B ligand in periodontal ligament cells |
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| المؤلفون: | Misa Ishiyama, Satoko Iwasa, Hisanori Ito, Masatake Asano, Tomokazu Hasegawa, Takashi Kifune, Tetsuo Shirakawa, Hiroki Takei |
| المصدر: | Journal of Oral Science. 60:544-551 |
| بيانات النشر: | Nihon University School of Dentistry, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Periodontal Ligament, Blotting, Western, Fluorescent Antibody Technique, Transfection, 03 medical and health sciences, 0302 clinical medicine, Osteoprotegerin, medicine, Humans, RNA, Messenger, RNA, Small Interfering, Hypoxia, General Dentistry, Cells, Cultured, Ccaat-enhancer-binding proteins, biology, Reverse Transcriptase Polymerase Chain Reaction, Activator (genetics), Chemistry, CCAAT-Enhancer-Binding Protein-beta, Monocyte, RANK Ligand, 030206 dentistry, Molecular biology, Amino Acids, Dicarboxylic, Resorption, 030104 developmental biology, medicine.anatomical_structure, RANKL, biology.protein, Cytokines, Tumor necrosis factor alpha, Macrophage migration inhibitory factor, Signal Transduction |
| الوصف: | Hypoxia after traumatic injuries to a tooth is one of the causes of subsequent root resorption. Inflammatory cytokines produced under hypoxic conditions are associated with root resorption, but the mechanism has not been fully understood. In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the regulation of CCAAT (cytosine-cytosine-adenosine-adenosine-thymidine)/enhancer-binding protein-β (C/EBPβ) and the receptor activator of nuclear factor kappa-B ligand (RANKL) expressions in immortalized human periodontal ligament (PDL) cells was investigated. PDL cells cultured under a hypoxic condition showed an increase in the expression of C/EBPβ and RANKL messenger RNAs (mRNAs), whereas the expression of osteoprotegerin and HIF-1α mRNAs was unaffected. Hypoxia had no effects on the secretion of interleukin (IL)-1β, IL-6, IL-8, IL-17A, tumor necrosis factor-alpha, macrophage migration inhibitory factor, monocyte chemoattractant protein-1, and macrophage colony-stimulating factor in the culture media. Treatment of the cells with dimethyloxaloylglycine, a competitive HIF prolyl hydroxylase inhibitor, significantly increased the expression of C/EBPβ and RANKL mRNAs. This suggested that the hypoxia-induced elevation of C/EBPβ and RANKL mRNAs was dependent on the HIF-1 activity. PDL cells transfected with a specific small interfering RNA designed to target the C/EBPβ gene showed a significant suppression of the RANKL mRNA. These findings indicated that C/EBPβ may play an important role in tooth root resorption via RANKL activation in hypoxia-exposed PDL cells. |
| تدمد: | 1880-4926 1343-4934 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb67dcc06ec229c6f4d85ba685d5cdb8Test https://doi.org/10.2334/josnusd.17-0436Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cb67dcc06ec229c6f4d85ba685d5cdb8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18804926 13434934 |
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