Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution
| العنوان: | Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution |
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| المؤلفون: | Arnold Szabo, Riccardo Panero, Rachel Cuttat, Simone Picelli, Pascal W. Hasler, Panagiotis Papasaikas, Cameron S. Cowan, Magdalena Renner, Roland Diggelmann, Akos Kusnyerik, Dinko Pavlinic, Florian Nigsch, Andreas Hierlemann, Botond Roska, Yanyan Hou, Tiago M. Rodrigues, Márton Balogh, Annick Waldt, Guglielmo Roma, Jacek Krol, Nadine Gerber-Hollbach, Hendrik P. N. Scholl, Patricia Galliker, Aldin Srdanovic, Michael B. Stadler, David Goldblum, Tamas Szikra, Stefan E. Spirig, Brigitte Gross-Scherf, Martin Munz, Sven Schuierer, Martina De Gennaro, Claudia P. Patino-Alvarez, Selim Orgül |
| المصدر: | Cell Cell, 182 (6) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Resource, Cell type, retina, macular degeneration, organoid, Cell, Induced Pluripotent Stem Cells, organoid development, Cell Culture Techniques, eye disease, Biology, General Biochemistry, Genetics and Molecular Biology, single cell sequencing, Cell Line, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine, Organoid, Humans, Genetic Predisposition to Disease, synaptic function, In Situ Hybridization, 030304 developmental biology, 0303 health sciences, Retina, retinal organoid, Gene Expression Regulation, Developmental, Retinal, Cell Differentiation, Epithelium, eye diseases, Cell biology, Electrophysiology, Organoids, Microscopy, Electron, medicine.anatomical_structure, Single cell sequencing, chemistry, Multigene Family, Synapses, Female, sense organs, human retina, Single-Cell Analysis, 030217 neurology & neurosurgery, Naphthoquinones |
| الوصف: | Summary Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic and translational research. We developed light-sensitive human retinal organoids with multiple nuclear and synaptic layers and functional synapses. We sequenced the RNA of 285,441 single cells from these organoids at seven developmental time points and from the periphery, fovea, pigment epithelium and choroid of light-responsive adult human retinas, and performed histochemistry. Cell types in organoids matured in vitro to a stable “developed” state at a rate similar to human retina development in vivo. Transcriptomes of organoid cell types converged toward the transcriptomes of adult peripheral retinal cell types. Expression of disease-associated genes was cell-type-specific in adult retina, and cell-type specificity was retained in organoids. We implicate unexpected cell types in diseases such as macular degeneration. This resource identifies cellular targets for studying disease mechanisms in organoids and for targeted repair in human retinas. Graphical Abstract Highlights • Light-sensitive, multilayered human retinal organoids with functional synapses • 285,441 transcriptomes from light-responsive human retinas and retinal organoids • Organoid cell types converge to adult peripheral retinal cell types • Linking retinal diseases to human retinal and retinal organoid cell types Light-sensitive, multilayered human retinal organoids with functional synapses are developed and benchmarked against human retinas obtained under conditions that preserve retinal integrity. |
| وصف الملف: | application/application/pdf |
| تدمد: | 1097-4172 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca36b60371a7ce7a3293aa2be695333eTest https://pubmed.ncbi.nlm.nih.gov/32946783Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ca36b60371a7ce7a3293aa2be695333e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974172 |
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