Effects of remote ischemic post-conditioning on fracture healing in rats
| العنوان: | Effects of remote ischemic post-conditioning on fracture healing in rats |
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| المؤلفون: | Zheng Li, Limin Liu, Guowei Lu, Huiliang Shen, Shuai An, Shibao Lu, Jiang Huang, Meng Zhou |
| المصدر: | Molecular Medicine Reports. 15:3186-3192 |
| بيانات النشر: | Spandidos Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, 0301 basic medicine, Cancer Research, Pathology, medicine.medical_specialty, Osteocalcin, Core Binding Factor Alpha 1 Subunit, Bone healing, Hindlimb, Biochemistry, Bone remodeling, Rats, Sprague-Dawley, Fractures, Bone, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Occlusion, Genetics, medicine, Animals, RNA, Messenger, Tibia, Ischemic Postconditioning, Molecular Biology, Fracture Healing, biology, business.industry, Osteoblast, X-Ray Microtomography, Alkaline Phosphatase, Hypoxia-Inducible Factor 1, alpha Subunit, Rats, Disease Models, Animal, Vascular endothelial growth factor A, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Oncology, biology.protein, Molecular Medicine, business, 030217 neurology & neurosurgery |
| الوصف: | Remote ischemic post‑conditioning (RIPC) is an established method to activate the hypoxia‑inducible factor‑1α (HIF‑1α) pathway, which is involved in the impairment of fracture healing. However, the role of RIPC in fracture healing remains to be fully elucidated. In the present study, rats received fractures and were divided into two groups: Control and RIPC, in which hind limb occlusion was performed. Rats were sacrificed at 7, 14, 28 and 42 days subsequent to tibial fracture. Micro‑computed tomography was performed to measure healing of the bone tissue and biomechanical testing was used to test mechanical strength. In addition, the effects of hind limb occlusion on the expression of two primary angiogenic mediators, HIF‑1α and vascular endothelial growth factor (VEGF), as well as the osteoblast markers runt‑related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin (OCN), were determined at the mRNA and protein levels by reverse transcription‑quantitative polymerase chain reaction, western blot analysis and immunohistochemistry. Systemic administration of hind limb occlusion (3 cycles/day, with each occlusion or release phase lasting 10 min) significantly promoted fracture healing and mechanical strength. The present study demonstrated that in rats treated with hind limb occlusion, the expression of HIF‑1α, VEGF, Runx2, ALP and OCN was significantly increased at the mRNA and protein levels, and that RIPC enhances fracture repair in vivo. |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9f2298dde0b286209c2c094311f65baTest https://doi.org/10.3892/mmr.2017.6348Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c9f2298dde0b286209c2c094311f65ba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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