Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells
| العنوان: | Staining Against Phospho-H2AX (gamma-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells |
|---|---|
| المؤلفون: | Paul N. Span, Anika Nagelkerke |
| المصدر: | Tumor Microenvironment: Study Protocols, pp. 1-10 Advances in Experimental Medicine and Biology ISBN: 9783319266640 Advances in Experimental Medicine and Biology ; 899, 1-10. Cham : Springer Int Publishing Ag STARTPAGE=1;ENDPAGE=10;TITLE=Advances in Experimental Medicine and Biology ; 899 |
| بيانات النشر: | Cham : Springer Int Publishing Ag, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Genome instability, medicine.diagnostic_test, DNA repair, DNA damage, cells, Molecular Materials, Biology, Immunofluorescence, environment and public health, Molecular biology, Staining, enzymes and coenzymes (carbohydrates), 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Histone, 030220 oncology & carcinogenesis, biology.protein, medicine, Immunohistochemistry, Advances in Experimental Medicine and Biology, biological phenomena, cell phenomena, and immunity, Immunostaining |
| الوصف: | Item does not contain fulltext Phospho-H2AX or gamma-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. Here, we provide a protocol to perform immunostaining for phospho-H2AX on cells, cryosections and formalin-fixed, paraffin-embedded tissues. Crucial steps in the protocol and troubleshooting suggestions are indicated. We also provide suggestions on how to combine staining against gamma-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels. |
| ردمك: | 978-3-319-26664-0 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9d31de93499f6a9ee4e87b04dd8082eTest https://hdl.handle.net/2066/166262Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....c9d31de93499f6a9ee4e87b04dd8082e |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9783319266640 |
|---|