Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
| العنوان: | Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome |
|---|---|
| المؤلفون: | Malin Fex, Åke Lernmark, Christiane S. Hampe, Mikaela Friedman, Gucci Jijuan Gu Urban, Ulf Landegren, Carina Törn, Masood Kamali-Moghaddam, Ping Ren |
| المصدر: | Scientific Reports; 5, no 11196 (2015) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, endocrine system diseases, Glutamate decarboxylase, Medical Biotechnology, Proximity ligation assay, Antigen-Antibody Complex, Stiff-Person Syndrome, Biology, Article, Immune system, immune system diseases, medicine, Humans, Medicinsk bioteknik, Aged, Autoantibodies, Immunoassay, Type 1 diabetes, Multidisciplinary, medicine.diagnostic_test, Glutamate Decarboxylase, Autoantibody, nutritional and metabolic diseases, Immunology in the medical area, Middle Aged, medicine.disease, Peptide Fragments, 3. Good health, Case-Control Studies, Immunology, Biomarker (medicine), Female, Stiff person syndrome |
| الوصف: | Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9a410d277fb59546e6fb4af9259331fTest https://lup.lub.lu.se/record/7485604Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c9a410d277fb59546e6fb4af9259331f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
|---|